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Availability
Please contact us to check the availability
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Immunogen
Recombinant Mammaglobin A, whole sequence (93 aa)
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Specificity
10 KDa Mammaglobin from human breast tissue
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Species reactivity
Human, Monkey, others not tested
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Product presentation
Affinity purified monoclonal antibody in stabilised phosphate buffer, containing 0.09 % sodium azide**. The volume is sufficient for app 250 immunohistochemical tests (100 µl working solution per test). For dilution we recommend our antibody diluent Art. No. PU002.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1
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Antibody s clone
304-1A5
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:50
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Protein concentration
50 µg/ml
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Positive control
Mamma carcinoma (normal mamma shows up lower expression of the protein), skin sweat glands
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Pretreatment
Formalin-fixed tissue may be pre-treated for 30 min at 100°C with Unmasking Solution C (Citrate buffer Art. No. DE000), E (buffered EDTA Art. No. DE006) or G (Glycol based solution Art. No. DE007) preferably in a pressure cooker.
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Tested applications
IHC, ELISA , WB
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Recommended secondary reagent
Gentaur Universal Staining Kits Art.-No. DA005 (AE005) or VECTASTAIN Elite ABC Mouse IgG (Art.-No. PK-6102) or VECTOR ImmPRESS™ Mouse IgG Kit (Art. No. MP-7402)
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Watson MA, Dintzis S, Darrow CM, Voss LE, DiPersio J, Jensen R, Fleming TP. Mammaglobin expression in primary, metastatic, and occult breast cancer. Cancer Res. 1999 Jul 1;59(13):3028-31. 2. Fanger GR, Houghton RL, Retter MW, Hendrickson RC, Babcook J, Dillon DC,Durham MD, Reynolds LD, Johnson JC, Carter D, Fleming TP, Roche PC, Persing DH, Reed SG. Detection of mammaglobin in the sera of patients with breast cancer. Tumour Biol. 2002 Jul-Aug;23(4):212-21. 3. Han JH, Kang Y, Shin HC, Kim HS, Kang YM, Kim YB, Oh SY. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med. 2003 Oct;127(10):1330-4. 4. Gillanders WE, Mikhitarian K, Hebert R, et al. Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis: an interim analysis of a prospective multi-institutional cohort study. Ann Surg. 2004 Jun;239(6):828-37; discussion 837-40.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus