Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph)

• Catalog number
  RPC20138
• Price
  Please ask
• Size
  200 μg

• Verified reactivity
  Escherichia coli (strain K12)
• Protein number
  P00557
• Gene number
  hph
• Other name
  APH(4)Hygromycin B phosphotransferaseHygromycin-B kinase
• Protein origin
  E.coli
• Protein region
  1-341aa
• Protein sequence
  MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
• Information about sequence
  Full Length
• Expected molecular weight
  54kDa
• Protein purity
  ≥ 90%
• Storage recommendation
  Aliquot and store at -20°C. Minimize freezing and thawing.
• Use before
  1 year
• Shipping requirements
  Blue ice
• Estimated production time
  7-11 business days
• Notes
  For research use only. Not for diagnostic procedures.
• Hyperlink
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Escherichia   coli   Hygromycin-B   4-O-kinase   hph  
• Gene symbol
  EBF4, CENPO, H4C15, POFUT1, SDR9C7, GNAO1, MIR1302-4, PIRC18
• Short name
  Recombinant Escherichia coli Hygromycin-B 4-O-kinase(hph)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. bioma advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  Escherichia coli
• Label
  N-terminal 6xHis-SUMO-tagged
• Alternative name
  Rec. Escherichia coli Hygromycin-B 4-O-phosphorylation catalyst(hph)
• Alternative technique
  rec, escherichia

Gene info

• Identity
  HGNC:29278
• Gene
  EBF4
• Long gene name
  EBF family member 4
• Synonyms gene name
  • early B cell factor 4
• Synonyms
  • KIAA1442
  • COE4
  • RP5-860F19.3
  • O/E-4
• GenBank acession
  • BC019106
• Locus
  20p13
• Discovery year
  2007-06-07
• Entrez gene record
  57593
• Pubmed identfication
  • 10718198
• RefSeq identity
  • NM_001110514
• Classification
  • Early B-cell factors
  • IPT domain containing
• VEGA ID
  OTTHUMG00000031709

Gene info

• Identity
  HGNC:28152
• Gene
  CENPO
• Long gene name
  centromere protein O
• Synonyms
  • MGC11266
  • CENP-O
• GenBank acession
  • AK027859
• Locus
  2p23.3
• Discovery year
  2006-06-15
• Entrez gene record
  79172
• Pubmed identfication
  • 16622420
  • 16622419
• RefSeq identity
  • NM_024322
• Classification
  • Constitutive centromere associated network
• VEGA ID
  OTTHUMG00000125525

Gene info

• Identity
  HGNC:29607
• Gene
  H4C15
• Long gene name
  H4 clustered histone 15
• Synonyms gene
  • HIST2H4B
• Synonyms gene name
  • histone 2, H4b
  • histone cluster 2, H4b
  • histone cluster 2 H4 family member b
• Synonyms
  • H4/o
• GenBank acession
  • BC108260
• Locus
  1q21.2
• Discovery year
  2006-06-12
• Entrez gene record
  554313
• Pubmed identfication
  • 15527963
• RefSeq identity
  • NM_001034077
• Classification
  • H4 histones
• VEGA ID
  OTTHUMG00000188201

Gene info

• Identity
  HGNC:14988
• Gene
  POFUT1
• Long gene name
  protein O-fucosyltransferase 1
• Synonyms
  • O-FUT
  • O-Fuc-T
  • KIAA0180
  • FUT12
• Synonyms name
  • peptide-O-fucosyltransferase
  • GDP-fucose protein O-fucosyltransferase 1
• GenBank acession
  • AF375884
• Locus
  20q11.21
• Discovery year
  2001-10-29
• Entrez gene record
  23509
• Pubmed identfication
  • 9023546
  • 9525914
• RefSeq identity
  • NM_015352
• Classification
  • MicroRNA protein coding host genes
  • Fucosyltransferases
• VEGA ID
  OTTHUMG00000133268

Gene info

• Identity
  HGNC:29958
• Gene
  SDR9C7
• Long gene name
  short chain dehydrogenase/reductase family 9C member 7
• Synonyms gene name
  • short chain dehydrogenase/reductase family 9C, member 7
• Synonyms
  • SDR-O
  • RDHS
• GenBank acession
  • AY044434
• Locus
  12q13.3
• Discovery year
  2008-12-22
• Entrez gene record
  121214
• Pubmed identfication
  • 12234675
  • 19027726
• RefSeq identity
  • NM_148897
• Classification
  • Short chain dehydrogenase/reductase superfamily
• VEGA ID
  OTTHUMG00000171004

Gene info

• Identity
  HGNC:4389
• Gene
  GNAO1
• Long gene name
  G protein subunit alpha o1
• Synonyms gene name
  • guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• Synonyms
  • G-ALPHA-o
• Locus
  16q13
• Discovery year
  1988-04-24
• Entrez gene record
  2775
• Pubmed identfication
  • 1899283
  • 11395521
• RefSeq identity
  • NM_020988
• Classification
  • G protein subunits alpha, group i
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000133241

Gene info

• Identity
  HGNC:35296
• Gene
  MIR1302-4
• Long gene name
  microRNA 1302-4
• Synonyms gene
  • MIRN1302-4
• Synonyms
  • hsa-mir-1302-4
• Locus
  2q33.3
• Discovery year
  2008-11-03
• Entrez gene record
  100302130
• RefSeq identity
  • NR_031633
• Classification
  • MicroRNA MIR1302 family

Gene info

• Identity
  HGNC:37522
• Gene
  PIRC18
• Long gene name
  piwi-interacting RNA cluster 18
• Locus
  4
• Discovery year
  2009-11-05
• Entrez gene record
  100313906
• Pubmed identfication
  • 17881367
• Classification
  • Piwi-interacting RNA clusters

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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