Protein A/G Agarose antibody Antibody Purification Reagent
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Catalog number
X1202
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Price
Please ask
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Size
25mL
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Category
Other Reagents
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Long description
Our recombinant Protein A and G combines the binding characteristics of Protein A and Protein G, providing an easy way to purify most immunoglobulins. Recombinant Protein A/G Agarose is produced by mixing equal amounts of our superior recombinant Protein A and Protein G agarose products.
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Antibody come from
n/a
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Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
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Clone
not specified
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Antigen antibody binding interaction
Protein A/G Agarose antibody Antibody Purification Reagent
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Antibody is raised in
see techfile
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Antibody Purification
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Antibody s suited for
Ligand Density: approximately 6 mg/ml of Protein A and 2 mg/ml of Protein G of drained gel _x000B_Dynamic Binding Capacity: approximately 18 to 35 mg human IgG per ml of drained gel (estimated in a 0.7x5cm column at a linear flow rate of 300 cm/h under the following conditions: binding buffer: 0.1 M PBS pH 7.0, Eluting buffer 0.1 M citrate pH 3.0. 2.8 times excess IgG of the binding capacity was applied. _x000B_Bead Structure: 4% highly cross-linked agarose _x000B_Bead Size Range: 45 to 165 µm _x000B_Mean Bead Size: approximately 90 µm _x000B_Maximum Linear Flow Rate: >1300 cm/hour at 25 deg C in an XK 16/20 column, bed height 5 cm _x000B_pH Stability: Long term storage - 3 to 9; short term storage - 2 to 10 _x000B_Chemical Stability: IgG binding capacity and recovery maintained after storage for (a) 7 days at 40¼ C in 3M NaSCN, 6M guanidine-HCl, 0.1M glycine (pH3.0), 70% ethanol; 10 hours at 23 ¼ C in 8M urea, 0.1M glycine (pH 11), 5.8M acetic acid (pH 2.0) _x000B_Physical stability: Negligible volume variation due to changes in pH or ionic strength _x000B_Sanitization: Sanitize the packed column with 2% Hibitane/20% ethanol or with 70% ethanol
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Storage
4ºC
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
The purest agarose was used in the production of Protein A/G Agarose antibody Antibody Purification Reagent by nordc. If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Conjugation
Agarose
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Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.
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French translation
anticorps
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Gene target
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Short name
Protein A/G Agarose antibody Antibody Purification Reagent
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Technique
Purification, Antibody, Agarose, agaroses, antibodies against human proteins, antibodies for, purified
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Isotype
not specified
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Label
unlabeled
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Alternative name
Protein A/G molecular sieve (antibody to-) (antibody to-) purification Reagent
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Alternative technique
purifications, antibodies, separation
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MeSH Data
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Name
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Concept
Scope note:
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
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Tree numbers
- E05.196.401.153
- E05.301.300.100
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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