Long description
Our recombinant Protein A and G combines the binding characteristics of Protein A and Protein G, providing an easy way to purify most immunoglobulins. Recombinant Protein A/G Agarose is produced by mixing equal amounts of our superior recombinant Protein A and Protein G agarose products.
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antigen-antibody binding interaction
Protein A/G Agarose antibody Antibody Purification Reagent
Antibody's reacts with
see techfile
Application
Antibody Purification
Antibody's suited for
Ligand Density: approximately 6 mg/ml of Protein A and 2 mg/ml of Protein G of drained gel _x000B_Dynamic Binding Capacity: approximately 18 to 35 mg human IgG per ml of drained gel (estimated in a 0.7x5cm column at a linear flow rate of 300 cm/h under the following conditions: binding buffer: 0.1 M PBS pH 7.0, Eluting buffer 0.1 M citrate pH 3.0. 2.8 times excess IgG of the binding capacity was applied. _x000B_Bead Structure: 4% highly cross-linked agarose _x000B_Bead Size Range: 45 to 165 µm _x000B_Mean Bead Size: approximately 90 µm _x000B_Maximum Linear Flow Rate: >1300 cm/hour at 25 deg C in an XK 16/20 column, bed height 5 cm _x000B_pH Stability: Long term storage - 3 to 9; short term storage - 2 to 10 _x000B_Chemical Stability: IgG binding capacity and recovery maintained after storage for (a) 7 days at 40¼ C in 3M NaSCN, 6M guanidine-HCl, 0.1M glycine (pH3.0), 70% ethanol; 10 hours at 23 ¼ C in 8M urea, 0.1M glycine (pH 11), 5.8M acetic acid (pH 2.0) _x000B_Physical stability: Negligible volume variation due to changes in pH or ionic strength _x000B_Sanitization: Sanitize the packed column with 2% Hibitane/20% ethanol or with 70% ethanol
Properties
The purest agarose was used in the production of Protein A/G Agarose antibody Antibody Purification Reagent by nordc.If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation
Agarose
Test
Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.
French translation
anticorps
Gene targetProtein
A/G
Antibody
Reagent
Short name
Protein A/G Agarose antibody Antibody Purification Reagent
Technique
Purification, Antibody, Agarose
Alternative name
Protein A/G molecular sieve (Antibody to) Antibody purification Reagent
