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Available ordering format
Lyophilized
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Immunogen
BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb1 proteins from angiosperms (monocots and dicots) and gymnosperms, includingArabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420 (Lhcb1.5)
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS01 004 | Lhcb1 | LHCII type I chlorophyll a/b-binding proteinAS01 004PRE | Lhcb1 | LHCII type I chlorophyll a/b-binding protein, pre-immune serumAS01 011 | 2 | Set of 10 plant anti-Lhca and anti-Lhcb antibodiesAS01 011 CHLAMYDOMONAS | 2 | Set of 4 Chlamydomonas anti-Lhc antibodiesPlant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1 : 2000, detected with standard ECL (WB)
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Molecular weight expected аpparent
28 | 25 kDa for Arabidopsis thaliana
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Verified reactivity
Arabidopsis thaliana, Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Rhoeo discolor, Silene vulgaris, Spinacia oleracea, Zea mays
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Possible reactivity
Triticum aestivum, Vitis vinifera, Brachypodium distachyon, Solanum tuberosum, Lotus japonicus, Cucumis sativus, Hordeum vulgare, Nicotiana tabacum, Aegilops tauschii, angiosperms (monocots and dicots), gymnosperms, mosses
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Protein is processed into mature form (Jansson 1999).
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References
Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015. Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675. Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794. Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.
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Scientific context
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476
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Notes
None
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Protein number
Q8VZ87 , P04777 , P04778 , Q9C5R6 , Q39141
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TAIR number
AT1G29910
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