FITC Isomer I; fluorescein-5-isothiocyanate
Soluble in: DMSO
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Laboratory chemicals *For Research Use Only*
Ambient (Room) temperature
The CAS or CASRN registry number for chemical substances and identifying chemicals by the Chemical Abstract Service is a worldwide used identification of chemicals. This chemical can be supplied in purities of 98% or 95% purity by aat. Bulk in kg or liter volumes on request.
This aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*
5-fluorecein [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*