anti-LEF1 Antibody
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Catalog number27-252
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PricePlease ask
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Size50 ug
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Gene infoLymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).
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Gene namesLEF1, DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
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Antibodie s labelunlabelled serum
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PurityAntibody is purified by peptide affinity chromatography method.
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Ab raised inRabbit
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TypePolyclonal
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AntigenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human LEF1.
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Immuno applicationELISA, Western Blot
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Antibody adviceLEF1 antibody can be used for detection of LEF1 by ELISA at 1:312500. LEF1 antibody can be used for detection of LEF1 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azideAntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep atFor short periods of storage (days) store at 4 grades C. For longer periods of storage, store LEF1 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works withHuman
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Omim nr7705917
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Ncbi nrNP_057353
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KDa44 kDa
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Vial with antibodyLyophilized
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Quantity per ml1 mg/ml
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NCBI GENE nr51176
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GENE symbolLEF1
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Antigene namelymphoid enhancer-binding factor 1
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SourceHomo sapiens
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Protein nrQ9UJU2
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolLEF1-AS1, LEF1
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Short nameanti-LEF1 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toLEF1 (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetlymphoid enhancer-binding factor 1, LEF1 and IDBG-33529 and ENSG00000138795 and 51176, DNA binding, nuclei, Lef1 and IDBG-194292 and ENSMUSG00000027985 and 16842, LEF1 and IDBG-640560 and ENSBTAG00000006844 and 535399
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Gene info
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Identity
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Gene
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Long gene nameLEF1 antisense RNA 1
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Synonyms gene name
- LEF1 antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2012-05-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelymphoid enhancer binding factor 1
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Synonyms gene name
- lymphoid enhancer-binding factor 1
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Synonyms
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Locus
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Discovery year1991-06-05
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Entrez gene record
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Pubmed identfication
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Classification
- TCF/LEF transcription factor family
- Wnt enhanceosome complex
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data