Goat Luciferase antibody (biotin)

Applications

ELISA, IHC, WB
Product Type

Primary Antibodies
Product Subtype

Conjugated Polyclonal Antibodies
Research Area

Proteases, Inhibitors, & Enzymes
Immunogen

Luciferase antibody (biotin) was raised in goat using highly purified firefly luciferase as the immunogen.
Specificity

Firefly
Cross Reactivity

Specific for firefly luciferase
Clone

NA
Concentration

50 ug/ml
Form Buffer

Affinity purified and supplied in PBS, pH 7.4, with 0.2% BSA.
Storage

Store at 4 DEG C for short term storage. Aliquot and store at -20 DEG C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping Info

Blue Ice
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
Latin name

Capra aegagrus hircus
French translation

anticorps

Gene target

Luciferase
Short name

Goat Luciferase antibody ( )
Technique

Antibody, Goat, antibodies against human proteins, antibodies for, goats
Host

Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
Isotype

NA
Label

luciferase
Species

Goats
Alternative name

Goat polyclonal Luciferase antibody (biotin) conjugated
Alternative technique

antibodies, caprine

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data