TGF-beta1, human recombinant
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Catalog number
4342-500
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Price
Please ask
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Size
500 ug
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Synonyms
Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor
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Alternative_names
Transforming Growth Factor-β1, Differentiation inhibiting factor, Cartilage-inducing factor
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Description
A protein that controls proliferation, cellular differentiation, & other functions in most cells
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Recombinant
Yes
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Source
CHO cells
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Purity
≥98%
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Biological activity
The ED₅₀ as determined by the ability of TGF-β1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is 2 x 10⁷ units/mg.
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Molecular Weight
25.0 kDa
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Storage Temp
-20°C
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Shipping
Gel pack
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Lyophilized from Sodium Citrate, pH3.5 buffer
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Reconstitution Instructions
Reconstitution in H₂O to a concentration of 50 ug/ml (e.g., 5 ug/100 ul) will yield a solution of 1 mM citrate buffer (pH 3.5). The solution can then be stored at 4°C for 1 month. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at –20°C.
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Background Information
TGF-β1 (Transforming Growth Factor-β1) is a member of the superfamily of homologous, disulfide-linked, homodimeric proteins that regulate the proliferation and differentiation of normal and transformed cells. Human TGF-β1 is a 25.0 kDa protein containing two identical 112 amino acid polypeptide chains linked by a single disulfide bond.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
LTBP1
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Short name
TGF-beta1, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
TGF-beta1, H. sapiens Rec.
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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