DYKDDDDK-Tag (3B9) mAb (Same as Sigma's Anti-FLAG)[DYKDDDDK-Tag (3B9)]
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Catalog number
MBS435047
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Price
Please ask
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Size
0.2 mL
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Products_type
Antibody
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Reactivity
General
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Conjugation
Flag
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Gene target
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Short name
DYKDDDDK-Tag (3B9) mAb (Same as Sigma's Anti- )[DYKDDDDK-Tag (3B9)]
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Technique
anti-, anti, antibody to, antibodies, Tags
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Host
Mouse
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Label
tag
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Alternative name
DYKDDDDK-detection labelled (3B9) monoclonal (Same as Sigma's Antibody toFLAG)[DYKDDDDK-detection labelled (3B9)]
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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