Cytochrome c Antibody
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Catalog number3025-100
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PricePlease ask
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Size100 µg
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Antibody TargetCytochrome c
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Target Alternative NameCYCS, CYC
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat, bovine, chicken, dog
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Immunogen SequenceSynthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
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AccessionNP_036972
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Gene Id25310
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AppearanceColorless liquid
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FormLiquid
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Concentration0.2 mg/ml
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Formulation100 µg (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation
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Application And UsagesWestern blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
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DescriptionCytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Dot Blot VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolCOX11, COX5A, COX17
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Short nameCytochrome c Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameCytochrome c (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namecytochrome c oxidase copper chaperone COX11
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Synonyms gene name
- COX11 (yeast) homolog, cytochrome c oxidase assembly protein
- cytochrome c oxidase assembly homolog 11 (yeast)
- COX11, cytochrome c oxidase copper chaperone
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-07-03
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial respiratory chain complex assembly factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome c oxidase subunit 5A
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Synonyms gene name
- cytochrome c oxidase subunit Va
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GenBank acession
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Locus
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Discovery year1998-12-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial complex IV: cytochrome c oxidase subunits
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecytochrome c oxidase copper chaperone COX17
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Synonyms gene name
- COX17 (yeast) homolog, cytochrome c oxidase assembly protein
- COX17 cytochrome c oxidase assembly homolog (S. cerevisiae)
- COX17, cytochrome c oxidase copper chaperone
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Mitochondrial respiratory chain complex assembly factors
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data