Cytochrome c Antibody

  • Catalog number
    3025-100
  • Price
    Please ask
  • Size
    100 µg
  • Antibody Target
    Cytochrome c
  • Target Alternative Name
    CYCS, CYC
  • Antibody Type
    Polyclonal
  • Species Reactivities
    Human, mouse, rat, bovine, chicken, dog
  • Immunogen Sequence
    Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c
  • Accession
    NP_036972
  • Gene Id
    25310
  • Appearance
    Colorless liquid
  • Form
    Liquid
  • Concentration
    0.2 mg/ml
  • Formulation
    100 µg (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin
  • Purification
    Affinity purified
  • Application
    Western blot, Immunoprecipitation
  • Application And Usages
    Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). The cytochrome c antibody detects the 12.6 kDa cytochrome c from human, mouse, and rat samples. Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysate can be used as positive controls.
  • Description
    Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases
  • Storage Condition
    -20 °C
  • Shipping Condition
    gel pack
  • Shelf Life
    12 months
  • Usage
    For Research Use Only! Not to be used in humans.
  • Handling
    The antibody solution should be gently mixed before use.
  • Western Blot Verified
    Verified
  • Immunocytochemistry Verified
    Not Verified
  • Immunofluorescence Verified
    Not Verified
  • Immunoprecipitation Verified
    Not Verified
  • FACS Verified
    Not Verified
  • ELISA Verified
    Not Verified
  • ChIP Verified
    Not Verified
  • Dot Blot Verified
    Not Verified
  • Flow Cytometry Verified
    Not Verified
  • Properties
    If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    COX11, COX5A, COX17
  • Short name
    Cytochrome c Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    Cytochrome c (antibody to-)
  • Alternative technique
    antibodies
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    cytochrome c oxidase subunit 5A
  • Synonyms gene name
    • cytochrome c oxidase subunit Va
  • GenBank acession
  • Locus
  • Discovery year
    1998-12-11
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Mitochondrial complex IV: cytochrome c oxidase subunits
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    cytochrome c oxidase copper chaperone COX17
  • Synonyms gene name
    • COX17 (yeast) homolog, cytochrome c oxidase assembly protein
    • COX17 cytochrome c oxidase assembly homolog (S. cerevisiae)
    • COX17, cytochrome c oxidase copper chaperone
  • GenBank acession
  • Locus
  • Discovery year
    1999-12-14
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Mitochondrial respiratory chain complex assembly factors
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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