Store Collagen III antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.
collagen, type III, alpha 1
Suspention, pH, azide
0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide.
Vial with antibody
ELISA, Western Blot, immuno-histo-chemistry, immuno precipitation
NCBI GENE nr
Collagen III antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 ug/mL.
Ab raised in
Collagen III antibody was made against Full length Collagen Type III with intact 3-dimensional helical structure from human and bovine placenta.
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
anti-Collagen III Antibody
antibody to-Collagen III (Antibody to)