Nampt (Visfatin/PBEF) (human) Intracellular ELISA Kit
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Catalog number
K4909-100
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Price
Please ask
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Size
100 assays
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Summary
• Detection method- Absorbance (450 nm) • Species reactivity- Mammalian • Applications-The assay detects Visfatin from serum samples in the range from 0.25 – 16 ng Visfatin/ml and a detection limit of 30 pg/ml.
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Applications
ELISA
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Sample Type
Cell lysates
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Features Benefits
• Simple procedure • Fast and convenient • High-throughput adaptable
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Storage Conditions
+4°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
Nampt, nicotinamide phosphoribosy-ltransferase, is the rate limiting enzyme of the mammalian NAD biosynthesis pathway. The circulating NMN (nicotinamide mononucleotide) and NAD are taken up by beta cells and converted to NAD by Nmnat (nicotinamide mononucleotide adenyltransferase) and intracellular Nampt. The Nampt plays a critical role in enhancing life span and protecting against oxidative damage. The Nampt (Visfatin/PBEF) (human) Intracelluar ELISA Kit is to be used for the in vitro quantitative determination of intracellular human Nampt (Visfatin/PBEF). This assay is a sandwich ELISA which utilizies a 96-well microtiter plate which was pre-coated with a monoclonal antibody and a purified polyclonal detection antibody. A HRP-conjugated anti-IgG and TMB (3,3’,5,5’-tetramethylbenzidine) is added to generate a color intensity directly proportional to the concentration of Nampt in the samples. This ELISA is specific for the measurement of natural and recombinant human Nampt. It does not cross-react with human adiponectin, human resistin, human RELM-β, human leptin, human GPX3, human ANGPTL4, human FABP4, human ANGPTL6, human PAI1. The assay range is 0.25 – 16 ng Nampt/ml and a detection limit of 30 pg/ml (based on adding two standard deviations to the mean value of the zero standard).
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
NAMPT
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Short name
Nampt (Visfatin/PBEF) ( ) Intracellular ELISA Kit
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Technique
intracellular, ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Human, Humans
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Alternative name
nicotinamide phosphoribosyltransferase (Visfatin/PBEF) (H. sapiens) Intracellular Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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