on ice packs/blue ice/+4 degrees C
Soluble in: Water
Wear appropriate gloves, protective clothing and eyewear and follow safe laboratory practices.
Laboratory chemicals *For Research Use Only*
To order FITC-streptavidin conjugate, please use the Cat. Nr.16910 and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
This aat Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aat FITC is highly sensitive to pH extremes.
fluorecein-biotinilated-binding streptavidin conjugate