Hepes

• Catalog number
  H998468
• Price
  Please ask
• Size
  500 mg

• Chemical available in other sizes
  Please inquire size and price
• Stock availability
  Please Inquire
• Cas number
  7365-45-9
• Chemical s molecular weight
  238.31
• Chemical s main applications
  Hepes
• Chemical s formula
  C8H18N2O4S
• Physical properties
  No Data Available
• Melting temperature
  No Data Available
• Boiling temperature
  No Data Available
• Chemical s soluble in
  No Data Available
• Stability conditions
  No Data Available
• Storage
  No Data Available
• Chemical s specifications
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Alternative name
  Hepes

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