Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1, Clone 5D9

  • Catalog number
    SMC-508D-A655
  • Price
    Please ask
  • Size
    100 µg
  • Clone
    5D9
  • Immunogen
    Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
  • Antibody s full description
    Mouse Anti- Hexanoyl-Lysine adduct Monoclonal IgG1 Antibody, Clone: 5D9: ATTO 655
  • Antibody s category
    Monoclonal Antibodies
  • Antibody s other name
    Hexanoyl-Lysine adduct Antibody, HEL (Hexanoyl-Lysine adduct) Antibody, HEL Antibody, HEL Adduct Antibody, Hexanoyl-Lys adduct Antibody, Hexanoyl-Lys Antibody, Hexanoyl-Lysine (HEL) adduct Antibody, Hexanoyl-Lys (HEL) Antibody, Hexanoyl Lysine adduct Antibody, Hexanoyl-Lysine adduct-modified protein Antibody, Hexanoyl-Lysine Adduct (HEL) Antibody
  • Raised in
    Mouse
  • Antibody s target
    Hexanoyl-Lysine adduct
  • Primary research fields
    Cancer, Oxidative Stress, Lipid peroxidation
  • Brandname
    MOLECULAR SIGNATURE®
  • Antibodies applications
    WB, ICC/IF, ELISA
  • Antibody s reactivity
    Species Independent
  • Antibody s dilutions
    WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
  • Purity
    Protein G Purified
  • Antibody buffer for storage
    PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins.
  • Storage recommendations
    -20ºC
  • Shipping recommendations
    Blue Ice or 4ºC
  • Antibody certificate of analysis
    A 1:1000 dilution of SMC-508 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
  • Antibody in cell
    See included datasheet or contact our support service
  • Tissue specificity
    See included datasheet or contact our support service
  • Released date
    23-Mar-2017
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • NCBI number
    Refer to NCBI
  • Gene number
    Refer to GenBank
  • Protein number
    Refer to Swiss-Prot
  • Antibody s datasheet
    Contact our support service
  • Representative figure link
  • Representative figure legend
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 (SMC-508). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (SMC-508) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250: 1:1000 for 30-60 min at RT. (A,C,E,F) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain (C,D) Phalloidin Alex Fluor 633 F-Actin stain (E,F) Hexanoyl-Lysine adduct Antibody (G,F) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl Lysine-BSA using Mouse Anti-Hexanoyl Lysine Monoclonal Antibody, Clone 5D9 (SMC-508). Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl Lysine Monoclonal Antibody (SMC-508) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa. Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of Hexanoyl lysine-BSA using Mouse Anti-Hexanoyl lysine Monoclonal Antibody, Clone 5D9 (SMC-508). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl lysine Monoclonal Antibody (SMC-508) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Mouse Anti-Hexanoyl-Lysine adduct Antibody [5D9] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Embryonic kidney cells (HEK293) (SMC-508) Mouse Anti-Hexanoyl-Lysine adduct Antibody [5D9] used in Western Blot (WB) on Hexanoyl Lysine-BSA Conjugate (SMC-508) Mouse Anti-Hexanoyl-Lysine adduct Antibody [5D9] used in Western Blot (WB) on Cervical Cancer cell line (HeLa) (SMC-508)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availability
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    ELAVL2, PATE3
  • Short name
    Mouse Anti-Hexanoyl-Lysine adduct (HEL) Monoclonal IgG1, Clone 5D9
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses
  • Host
    mouse
  • Isotype
    IgG1, IgG1
  • Label
    ATTO 655
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse Antibody toHexanoyl-Lysine adduct (HEL) monoclonal IgG1, clonality 5D9
  • Alternative technique
    murine, antibodies
  • Clone name
    5D9
Gene info
  • Identity
  • Gene
  • Long gene name
    ELAV like RNA binding protein 2
  • Synonyms gene name
    • ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2
    • ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
    • ELAV like neuron-specific RNA binding protein 2
  • Synonyms
  • Synonyms name
  • GenBank acession
  • Locus
  • Discovery year
    1996-11-15
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • RNA binding motif containing
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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