FGF Basic Recombinant Protein
-
Catalog number
40-480
-
Price
Please ask
-
Size
0.01 mg, 0.05 mg
-
-
About recombinant protein
FGF-basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF-basic signals through FGFR 1b, 1c, 2c, 3c and 4. Recombinant rat FGF-basic is a 16.3 kDa protein consisting of 145 amino acid residues.
-
Other name of the recombinant
bFGF, Fgf-2, Fibroblast growth factor 2, Basic fibroblast growth factor, FGF-2
-
Recombinant in
E. coli
-
SEQUENCE
PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
-
Activity
The ED50 was determined by the dose - dependent stimulation of thymidine uptake by 3T3 cells is ≤ 0.2 ng/mL corresponding to a specific activity of ≥5 x 106 units/mg.
-
Keep at
The lyophilized FGF Basic recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted FGF Basic is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing FGF Basic recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
-
Rec PROTEIN GI
9506599
-
Accession nr
NP_062178.1
-
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
-
About
This product is for research use only.
-
Protein s origin
Rat
-
Vial
Lyophilized
-
NCBI ID
54250
-
Gene name
fibroblast growth factor 2
-
Source
Rattus norvegicus
-
Uniprot
P13109
-
-
-
Description
Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
Fgf2
-
Short name
FGF Recombinant Protein
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative name
FGF Basic Rec. Protein
-
Alternative technique
rec
-
Gene info
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products
