Carbonic Anhydrase 3, human recombinant

• Catalog number
  7833-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  CA3, Carbonic anhydrase 3
• Alternative_names
  CA3, Carbonic anhydrase 3
• Description
  Catalyzes the reversible hydration of carbon dioxide
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Activity Specifications test method
  Measured by its esterase activity. The specific activity is >1 pmol/min/µg.
• Biological activity
  Measured by its esterase activity. The specific activity is >1 pmol/min/µg.
• Results
  The specific activity is >1 pmol/min/µg.
• Molecular Weight
  This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 30.4 kDa. The predicted N-terminus is Met 1. DTT-reduced Protein migrates as 28-30 kDa in SDS-PAGE.
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.
• Background Information
  Carbonic anhydrase 3 (CA3) is also known as Carbonate dehydratase III, Carbonic anhydrase III, which belongs to the alpha-carbonic anhydrase family. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The catalytic activity of carbonic anhydrase 3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Carbonic   Anhydrase  
• Gene symbol
  CA3, CA15P3
• Short name
  Carbonic Anhydrase 3, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Carbonic Anhydrase 3, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:1374
• Gene
  CA3
• Long gene name
  carbonic anhydrase 3
• Synonyms gene name
  • carbonic anhydrase III, muscle specific
  • carbonic anhydrase III
• Synonyms
  • Car3
  • CAIII
• GenBank acession
  • AJ006473
• Locus
  8q21.2
• Discovery year
  2001-06-22
• Entrez gene record
  761
• Pubmed identfication
  • 6221502
• RefSeq identity
  • NM_005181
• Classification
  • Carbonic anhydrases
• VEGA ID
  OTTHUMG00000164942

Gene info

• Identity
  HGNC:44363
• Gene
  CA15P3
• Long gene name
  CA15 pseudogene 3
• Synonyms gene name
  • carbonic anhydrase XV, pseudogene 3
• Locus
  22q11.21
• Discovery year
  2012-08-21
• Entrez gene record
  100996556
• Pubmed identfication
  • 16083424
• Classification
  • Carbonic anhydrases

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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