Mouse anti Reticulon-1C / NSP-C

• Catalog number
  MUB1315S
• Price
  Please ask
• Size
  1 mL

• Category
  Primary Antibodies
• Long description
  Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
• Antibody come from
  RNL-4 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells, with spleen cells from a BALB/c Mouse immunized with a synthetic peptide encompassing the unique 20 N-terminal amino acid sequence of Reticulon-1C.
• Other description
  Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
• Clone
  RNL-4
• Antigen antibody binding interaction
  Mouse anti Reticulon-1C / NSP-C Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Human,Rat,Swine
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  RNL-4 recognizes an epitope loCated within the first 20 amino acids of Reticulon-1C (NSP-C). RNL-4 reacts with peripheral nerves and ganglia of various tissues and cross-reacts with smooth muscle cells and myoepithelium. In the central nervous system it reacts with the neurohypophysis and pars intermedia of the pituitary gland, and a weak diffuse staining was observed in neurons of the granular and molecular layer of the cerebellar cortex, while glial cells, cerebellar medulla and Purkinje cells are negative. Reticulon-1 has been found to indiCate neuronal differentiation and to be downregulated in neurological pathologies.
• Research interest
  Cancer,Neurobiology
• Application
  Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Western blotting
• Antibody s suited for
  RNL-4 is useful for immunocytochemistry, immunohistochemistry on frozen tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:2 – 1:10 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:50 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Senden, N. H., Timmer, E. D., Boers, J. E., van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1996). Neuroendocrine-specific protein C (NSP-C): subcellular localization and differential expression in relation to NSP-A, Eur J Cell Biol 69, 197-213. Senden, N. H., Timmer, E. D., de Bruine, A., Wagenaar, S. S., Van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1997). A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers, J Pathol 182, 13-21. Hens, J., Nuydens, R., Geerts, H., Senden, N. H., Van de Ven, W. J., Roebroek, A. J., van de Velde, H. J., Ramaekers, F. C., and Broers, J. L. (1998). Neuronal differentiation is accompanied by NSP-C expression, Cell Tissue Res 292, 229-37. Kim, S. H., Yoo, B. C., Broers, J. L., Cairns, N., and Lubec, G. (2000). Neuroendocrine-specific protein C, a marker of neuronal differentiation, is reduced in brain of patients with Down syndrome and Alzheimer's disease, Biochem Biophys Res Commun 276, 329-34.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Reticulon-1C   NSP-C  
• Gene symbol
  MARK1, SREBF1, TFCP2, PTPN6, RBM8A, RTN1, SMC4, PCK1, MYB, RTN4
• Short name
  Mouse anti Reticulon-1C / NSP-C
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Reticulon-1C / NSP-C
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:6896
• Gene
  MARK1
• Long gene name
  microtubule affinity regulating kinase 1
• Synonyms gene name
  • MAP/microtubule affinity-regulating kinase 1
• Synonyms
  • MARK
  • PAR-1C
• GenBank acession
  • AF154845
• Locus
  1q41
• Discovery year
  1997-10-27
• Entrez gene record
  4139
• Pubmed identfication
  • 9108484
• VEGA ID
  OTTHUMG00000037351

Gene info

• Identity
  HGNC:11289
• Gene
  SREBF1
• Long gene name
  sterol regulatory element binding transcription factor 1
• Synonyms
  • SREBP1
  • bHLHd1
  • SREBP-1c
  • SREBP1a
• GenBank acession
  • BC057388
• Locus
  17p11.2
• Discovery year
  1994-11-23
• Entrez gene record
  6720
• Pubmed identfication
  • 8402897
  • 7759101
• RefSeq identity
  • NM_004176
• Classification
  • Basic helix-loop-helix proteins
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000059313

Gene info

• Identity
  HGNC:11748
• Gene
  TFCP2
• Long gene name
  transcription factor CP2
• Synonyms
  • CP2
  • LSF
  • LBP-1C
  • TFCP2C
• GenBank acession
  • U03494
• Locus
  12q13.12-q13.13
• Discovery year
  1994-12-15
• Entrez gene record
  7024
• Pubmed identfication
  • 8157699
• RefSeq identity
  • NM_005653
• VEGA ID
  OTTHUMG00000169621

Gene info

• Identity
  HGNC:9658
• Gene
  PTPN6
• Long gene name
  protein tyrosine phosphatase non-receptor type 6
• Synonyms
  • HCP
  • HCPH
  • PTP-1C
  • SHP-1
  • SHP1
• Locus
  12p13.31
• Discovery year
  1992-02-12
• Entrez gene record
  5777
• Pubmed identfication
  • 1639416
• RefSeq identity
  • NM_002831
• Classification
  • Protein tyrosine phosphatases non-receptor type
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000168518

Gene info

• Identity
  HGNC:9905
• Gene
  RBM8A
• Long gene name
  RNA binding motif protein 8A
• Synonyms gene
  • RBM8
• Synonyms
  • ZNRP
  • BOV-1A
  • BOV-1B
  • BOV-1C
  • RBM8B
  • Y14
• Synonyms name
  • binder of OVCA1
• GenBank acession
  • AF127761
• Locus
  1q21.1
• Discovery year
  1999-05-05
• Entrez gene record
  9939
• Pubmed identfication
  • 11004516
  • 11013075
• RefSeq identity
  • NM_005105
• Classification
  • Spliceosomal P complex
  • RNA binding motif containing
  • Exon junction complex
• VEGA ID
  OTTHUMG00000013736
• Locus Specific Databases
  • LRG_574

Gene info

• Identity
  HGNC:10467
• Gene
  RTN1
• Long gene name
  reticulon 1
• Synonyms gene
  • NSP
• Synonyms gene name
  • neuroendocrine-specific protein
• GenBank acession
  • L10333
• Locus
  14q23.1
• Discovery year
  1993-08-24
• Entrez gene record
  6252
• Pubmed identfication
  • 8275708
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000028947

Gene info

• Identity
  HGNC:14013
• Gene
  SMC4
• Long gene name
  structural maintenance of chromosomes 4
• Synonyms gene
  • SMC4L1
• Synonyms gene name
  • SMC4 (structural maintenance of chromosomes 4, yeast)-like 1
  • SMC4 structural maintenance of chromosomes 4-like 1 (yeast)
• Synonyms
  • hCAP-C
  • CAP-C
• GenBank acession
  • AF092564
• Locus
  3q25.33
• Discovery year
  2001-02-22
• Entrez gene record
  10051
• Pubmed identfication
  • 9789013
  • 10319587
• Classification
  • Structural maintenance of chromosomes proteins
  • Condensin I subunits
  • Condensin II subunits
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000159006

Gene info

• Identity
  HGNC:8724
• Gene
  PCK1
• Long gene name
  phosphoenolpyruvate carboxykinase 1
• Synonyms gene name
  • phosphoenolpyruvate carboxykinase 1 (soluble)
• Synonyms
  • PEPCK-C
• Locus
  20q13.31
• Discovery year
  1990-09-10
• Entrez gene record
  5105
• Pubmed identfication
  • 1492743
• VEGA ID
  OTTHUMG00000032825

Gene info

• Identity
  HGNC:7545
• Gene
  MYB
• Long gene name
  MYB proto-oncogene, transcription factor
• Synonyms gene name
  • v-myb avian myeloblastosis viral oncogene homolog
• Synonyms
  • c-myb
• Locus
  6q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  4602
• Pubmed identfication
  • 17599807
• Classification
  • Myb/SANT domain containing
• VEGA ID
  OTTHUMG00000015629
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:14085
• Gene
  RTN4
• Long gene name
  reticulon 4
• Synonyms
  • NSP-CL
  • KIAA0886
  • NOGO
  • ASY
• GenBank acession
  • AF087901
• Locus
  2p16.1
• Discovery year
  2000-11-28
• Entrez gene record
  57142
• Pubmed identfication
  • 10667797
  • 10773680
• VEGA ID
  OTTHUMG00000129337

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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