Mouse anti Reticulon-1A / NSP-A
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Catalog numberMUB1311P
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PricePlease ask
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Size0,1 mg
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CategoryPrimary Antibodies
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Long descriptionRecently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
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Antibody come fromMON-161 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (βGAL-NSP-A 6-776).
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Other descriptionEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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CloneMON161
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Antigen antibody binding interactionMouse anti Reticulon-1A / NSP-A Antibody
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Antibody is raised inMouse
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Antibody s reacts withHamster,Human,Mouse,Rat
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityMON-161 exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-161 is loCated between amino acid residues 174-337 of Reticulon-1A.
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Research interestCancer,Neurobiology
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ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
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Antibody s suited forMON-161 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissue, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:50 – 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:50 – 1:500 for immunoblotting applications.
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StorageStore at 4°C, or in small aliquots at -20°C.
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Relevant referencesRoebroek, A.J.M., van de Velde, H.J.K., Van Bokhoven, A., Broers, J.L.V., Ramaekers, F.C.S., Van de Ven, W.J.M. (1993). Cloning and expression of alternative transcripts of a noval neuroendocrine-specific gene and identifiCation of its 135-kDa translation product. J. Biol. Chem. 268,13439-47. van de Velde, H. J., Roebroek, A. J., van Leeuwen, F. W., and Van de Ven, W. J. (1994). Molecular analysis of expression in Rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum, Mol Brain Res 23, 81-92. van de Velde, H. J., Roebroek, A. J., Senden, N. H., Ramaekers, F. C., and Van de Ven, W. J. (1994). NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum, J Cell Sci 107, 2403-16. van de Velde, H. J., Senden, N. H., Roskams, T. A., Broers, J. L., Ramaekers, F. C., Roebroek, A. J., and Van de Ven, W. J. (1994). NSP-encoded reticulons are neuroendocrine markers of a novel Category in Human lung cancer diagnosis, Cancer Res 54, 4769-76. Senden, N. H., Timmer, E. D., de Bruine, A., Wagenaar, S. S., Van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1997). A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers, J Pathol 182, 13-21 Hens, J., Nuydens, R., Geerts, H., Senden, N. H., Van de Ven, W. J., Roebroek, A. J., van de Velde, H. J., Ramaekers, F. C., and Broers, J. L. (1998). Neuronal differentiation is accompanied by NSP-C expression, Cell Tissue Res 292, 229-37.
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHD1OR15-1A, H2BC8, UBP1, MTNR1A, IGKV2OR2-1, SCLT1, APH1A, HIF1A-AS1, HIF1A-AS2, MARK3
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Short nameMouse anti Reticulon-1A / NSP-A
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TechniqueMouse, anti, antibody to, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to Reticulon-1A / NSP-A
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Alternative techniquemurine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy diversity 1/OR15-1A (non-functional)
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Synonyms gene name
- immunoglobulin heavy diversity 1/OR15-1A
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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Classification
- Immunoglobulin heavy (IGH) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameH2B clustered histone 8
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Synonyms gene
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Synonyms gene name
- H2B histone family, member A
- histone 1, H2bg
- histone cluster 1, H2bg
- histone cluster 1 H2B family member g
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- H2B histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameupstream binding protein 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-05-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemelatonin receptor 1A
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Synonyms
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Locus
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Discovery year1995-03-22
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Entrez gene record
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Pubmed identfication
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Classification
- Melatonin receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 2/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 2/OR2-1
- immunoglobulin kappa variable 2/OR2-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namesodium channel and clathrin linker 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-07-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameaph-1 homolog A, gamma-secretase subunit
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Synonyms gene name
- anterior pharynx defective 1 homolog A (C. elegans)
- APH1A gamma secretase subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-02-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHIF1A antisense RNA 1
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Synonyms gene name
- HIF1A antisense RNA 1 (non-protein coding)
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Synonyms
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GenBank acession
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Locus
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Discovery year2011-09-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHIF1A antisense RNA 2
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Synonyms gene name
- HIF1A antisense RNA 2 (non-protein coding)
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Synonyms
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Locus
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Discovery year2011-09-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicrotubule affinity regulating kinase 3
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Synonyms gene name
- MAP/microtubule affinity-regulating kinase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-10-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data