Mouse anti Reticulon-1A / NSP-A

• Catalog number
  MUB1311P
• Price
  Please ask
• Size
  0,1 mg

• Category
  Primary Antibodies
• Long description
  Recently, a novel gene family has been identified and characterized, designated the Reticulons because the proteins encoded by these genes are anchored to the membranes of the endoplasmic reticulum. Reticulon-1 was formerly designated NSP for Neuroendocrine-Specific-Protein, because it is specifically expressed in neural and neuroendocrine tissues. The NSP-gene has been mapped by fluorescence in situ hybridization to Human chromosome 14q21-q22. The NSP-gene encodes three overlapping proteins, i.e. Reticulon-1A (NSP-A), Reticulon-1B (NSP-B), and Reticulon-1C (NSP-C). These proteins were found to be anchored to membranes of the endoplasmic reticulum through their common carboxy-terminal regions. Reticulon-1A is a protein with a molecular weight (MW) of about 135 kDa, which occurs in various isoforms presumably depending on the degree of phosphorylation of serine residues. In lung cancer diagnosis Reticulon-1A appeared to be a reliable marker for the detection of neuroendocrine differentiation, since most of the small cell lung carcinoma (SCLC) and carcinoid tumors showed expression of Reticulon-1A. Reticulon-1B is a phosphoprotein with a MW of 45 kDa and is restricted to the lung cancer cell line NCI-H82. Reticulon-1B is sofar not found in Human tissues. Reticulon-1C is a protein with a MW of 23 kDa which is not phosphorylated and is found with Reticulon-1A in SCLC (cell lines) and not in non-SCLC (cell cultures).
• Antibody come from
  MON-161 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (βGAL-NSP-A 6-776).
• Other description
  Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
• Clone
  MON161
• Antigen antibody binding interaction
  Mouse anti Reticulon-1A / NSP-A Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  Hamster,Human,Mouse,Rat
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  MON-161 exclusively recognizes the 135 kD Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues. The epitope of MON-161 is loCated between amino acid residues 174-337 of Reticulon-1A.
• Research interest
  Cancer,Neurobiology
• Application
  Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
• Antibody s suited for
  MON-161 is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissue, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:50 – 1:100 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:50 – 1:500 for immunoblotting applications.
• Storage
  Store at 4°C, or in small aliquots at -20°C.
• Relevant references
  Roebroek, A.J.M., van de Velde, H.J.K., Van Bokhoven, A., Broers, J.L.V., Ramaekers, F.C.S., Van de Ven, W.J.M. (1993). Cloning and expression of alternative transcripts of a noval neuroendocrine-specific gene and identifiCation of its 135-kDa translation product. J. Biol. Chem. 268,13439-47. van de Velde, H. J., Roebroek, A. J., van Leeuwen, F. W., and Van de Ven, W. J. (1994). Molecular analysis of expression in Rat brain of NSP-A, a novel neuroendocrine-specific protein of the endoplasmic reticulum, Mol Brain Res 23, 81-92. van de Velde, H. J., Roebroek, A. J., Senden, N. H., Ramaekers, F. C., and Van de Ven, W. J. (1994). NSP-encoded reticulons, neuroendocrine proteins of a novel gene family associated with membranes of the endoplasmic reticulum, J Cell Sci 107, 2403-16. van de Velde, H. J., Senden, N. H., Roskams, T. A., Broers, J. L., Ramaekers, F. C., Roebroek, A. J., and Van de Ven, W. J. (1994). NSP-encoded reticulons are neuroendocrine markers of a novel Category in Human lung cancer diagnosis, Cancer Res 54, 4769-76. Senden, N. H., Timmer, E. D., de Bruine, A., Wagenaar, S. S., Van de Velde, H. J., Roebroek, A. J., Van de Ven, W. J., Broers, J. L., and Ramaekers, F. C. (1997). A comparison of NSP-reticulons with conventional neuroendocrine markers in immunophenotyping of lung cancers, J Pathol 182, 13-21 Hens, J., Nuydens, R., Geerts, H., Senden, N. H., Van de Ven, W. J., Roebroek, A. J., van de Velde, H. J., Ramaekers, F. C., and Broers, J. L. (1998). Neuronal differentiation is accompanied by NSP-C expression, Cell Tissue Res 292, 229-37.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Reticulon-1A   NSP-A  
• Gene symbol
  IGHD1OR15-1A, H2BC8, UBP1, MTNR1A, IGKV2OR2-1, SCLT1, APH1A, HIF1A-AS1, HIF1A-AS2, MARK3
• Short name
  Mouse anti Reticulon-1A / NSP-A
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to Reticulon-1A / NSP-A
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:5487
• Gene
  IGHD1OR15-1A
• Long gene name
  immunoglobulin heavy diversity 1/OR15-1A (non-functional)
• Synonyms gene name
  • immunoglobulin heavy diversity 1/OR15-1A
• Synonyms
  • IGHD1/OR15-1A
  • IGHD1OR151A
• GenBank acession
  • X55575
• Locus
  15q11.2
• Discovery year
  2000-04-17
• Entrez gene record
  28335
• Classification
  • Immunoglobulin heavy (IGH) orphons
• VEGA ID
  OTTHUMG00000184580

Gene info

• Identity
  HGNC:4746
• Gene
  H2BC8
• Long gene name
  H2B clustered histone 8
• Synonyms gene
  • H2BFA
  • HIST1H2BG
• Synonyms gene name
  • H2B histone family, member A
  • histone 1, H2bg
  • histone cluster 1, H2bg
  • histone cluster 1 H2B family member g
• Synonyms
  • H2B/a
  • H2B.1A
• GenBank acession
  • M60750
• Locus
  6p22.2
• Discovery year
  1998-08-27
• Entrez gene record
  8339
• Pubmed identfication
  • 1916825
  • 12408966
• RefSeq identity
  • NM_003518
• Classification
  • H2B histones
• VEGA ID
  OTTHUMG00000014446

Gene info

• Identity
  HGNC:12507
• Gene
  UBP1
• Long gene name
  upstream binding protein 1
• Synonyms
  • LBP-1a
• GenBank acession
  • AF198487
• Locus
  3p22.3
• Discovery year
  1996-05-13
• Entrez gene record
  7342
• Pubmed identfication
  • 8114710
• RefSeq identity
  • NM_014517
• VEGA ID
  OTTHUMG00000130749

Gene info

• Identity
  HGNC:7463
• Gene
  MTNR1A
• Long gene name
  melatonin receptor 1A
• Synonyms
  • MEL-1A-R
• Locus
  4q35.2
• Discovery year
  1995-03-22
• Entrez gene record
  4543
• Pubmed identfication
  • 7558006
• Classification
  • Melatonin receptors
• VEGA ID
  OTTHUMG00000185575

Gene info

• Identity
  HGNC:5805
• Gene
  IGKV2OR2-1
• Long gene name
  immunoglobulin kappa variable 2/OR2-1 (pseudogene)
• Synonyms gene
  • IGKV2OR2-1A
• Synonyms gene name
  • immunoglobulin kappa variable 2/OR2-1
  • immunoglobulin kappa variable 2/OR2-1 pseudogene
• Synonyms
  • IGKV2/OR2-1
  • IGKV2/OR2-1A
• GenBank acession
  • X05101
• Locus
  2q11.2
• Discovery year
  2000-04-18
• Entrez gene record
  28861
• Pubmed identfication
  • 8011175
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155332

Gene info

• Identity
  HGNC:26406
• Gene
  SCLT1
• Long gene name
  sodium channel and clathrin linker 1
• Synonyms
  • hCAP-1A
  • FLJ30655
• GenBank acession
  • AK055217
• Locus
  4q28.2
• Discovery year
  2006-07-18
• Entrez gene record
  132320
• Pubmed identfication
  • 15797711
• RefSeq identity
  • NM_144643
• VEGA ID
  OTTHUMG00000133346

Gene info

• Identity
  HGNC:29509
• Gene
  APH1A
• Long gene name
  aph-1 homolog A, gamma-secretase subunit
• Synonyms gene name
  • anterior pharynx defective 1 homolog A (C. elegans)
  • APH1A gamma secretase subunit
• Synonyms
  • APH-1A
  • CGI-78
• GenBank acession
  • AF151835
• Locus
  1q21.2
• Discovery year
  2005-02-08
• Entrez gene record
  51107
• Pubmed identfication
  • 10810093
  • 12110170
• RefSeq identity
  • NM_016022
• VEGA ID
  OTTHUMG00000012545

Gene info

• Identity
  HGNC:43014
• Gene
  HIF1A-AS1
• Long gene name
  HIF1A antisense RNA 1
• Synonyms gene name
  • HIF1A antisense RNA 1 (non-protein coding)
• Synonyms
  • 5'aHIF-1A
• GenBank acession
  • CN270307
• Locus
  14q23.2
• Discovery year
  2011-09-16
• Entrez gene record
  100750246
• Pubmed identfication
  • 21897117
  • 24875884
• RefSeq identity
  • NR_047116
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000171134

Gene info

• Identity
  HGNC:43015
• Gene
  HIF1A-AS2
• Long gene name
  HIF1A antisense RNA 2
• Synonyms gene name
  • HIF1A antisense RNA 2 (non-protein coding)
• Synonyms
  • 3'aHIF-1A
  • aHIF
• Locus
  14q23.2
• Discovery year
  2011-09-16
• Entrez gene record
  100750247
• Pubmed identfication
  • 21897117
  • 27018306
• RefSeq identity
  • NR_045406
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000171135

Gene info

• Identity
  HGNC:6897
• Gene
  MARK3
• Long gene name
  microtubule affinity regulating kinase 3
• Synonyms gene name
  • MAP/microtubule affinity-regulating kinase 3
• Synonyms
  • CTAK1
  • KP78
  • PAR-1A
• GenBank acession
  • M80359
• Locus
  14q32.32-q32.33
• Discovery year
  1997-10-27
• Entrez gene record
  4140
• Pubmed identfication
  • 9533022
• RefSeq identity
  • NM_001128918
• VEGA ID
  OTTHUMG00000171789

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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