Fusion protein amino acids 1-524 (full length) of human EAAC1
Antibody's other name
Excitatory amino-acid carrier 1 Antibody, excitatory amino acid transporter 3 Antibody, EAAT3 Antibody, Neuronal and epithelial glutamate transporter Antibody, Sodium-dependent glutamate/aspartate transporter 3 Antibody, solute carrier family 1 member 1 and Slc1a1 Antibody
Primary research fields
Neuroscience, Cell Structure, Membrane Markers, Pumps/Transporters, Neurotransmitter Receptors
WB, IP, ICC/IF
WB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
Protein G Purified
Antibody buffer for storage
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Blue Ice or 4ºC
Antibody certificate of analysis
5 µg of SMC-406 was sufficient for immunoprecipitation of EAAC1 from 50 µg of rat brain lysate.
Antibody in cell
See included datasheet or contact our support service
SLC1A1/EAAT3/EAAC1, a member of this transporter family, is expressed throughout the body, particularly in brain, intestine, liver, heart and kidney. Functional defects of the SLC1A1 gene are found in patients of dicarboxylicamino aciduria.
1. Smith C. P., et al., (1994) Genomics 20:335-336. 2. Kanai Y., and Hediger M. A. (1992) Nature 360:467-471.
To be tested
To be tested
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Representative figure legend
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-EAAC1 Monoclonal Antibody, Clone S180-41 (SMC-406). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-EAAC1 Monoclonal Antibody (SMC-406) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Membrane . Magnification: 60X. Western Blot analysis of Rat brain membrane lysate showing detection of EAAT3 protein using Mouse Anti-EAAT3 Monoclonal Antibody, Clone S180-41 (SMC-406). Primary Antibody: Mouse Anti-EAAT3 Monoclonal Antibody (SMC-406) at 1:1000. Mouse Anti-EAAC1 Antibody [S180-41] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SMC-406) Mouse Anti-EAAT3 Antibody [S180-41] used in Western Blot (WB) on Rat brain membrane lysate (SMC-406)
Country of production
Total weight (kg)
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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