Procathepsin K, human recombinant
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Catalog number
1026-1000
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Price
Please ask
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Size
1 mg
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Synonyms
CTSK, CTSO, CTSO3
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Alternative_names
CTSK, CTSO, CTSO3
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Description
Predominant proteinase responsible for the resorption of the bone matrix.
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Recombinant
Yes
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Source
E. coli.
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
The specific activity is >1000 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)
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Results
The specific activity is >1000 mU/mg (1U = 1 µmole/min/mg) as measured by Cathepsin K Activity Assay Kit (Catalog #K141-100)
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Unit Definition
1U = 1 µmole/min/mg
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Molecular Weight
35.9 kDa
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
12 months
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Concentration
1 mg/ml
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Appearance
Liquid
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Physical form description
1 mg/ml solution in 25 mM Na2HPO4 and 500 mM NaCl (pH 7.0).
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Background Information
Cathepsin K, a member of the papain cysteine proteinase family is the predominant proteinase responsible for the resorption of the bone matrix. Cathepsin cleaves proteins such as collagen type I, collagen type II and osteonectin, thereby playing a role in bone remodeling and resorption in osteoporosis, osteolytic bone metastasis and rheumatoid arthritis (Bromme and Okamoto, 1995; Drake, F. et al 1996; Bossard et al, 1996). Cathepsin K is synthesized as an inactive proenzyme (35.1 kDa) that is converted to its mature active form (23.6 kDa) by proteolytic cleavage of its 99-amino-acid propeptide domain. The in-vitro processing of procathepsin K to mature cathepsin K is autocatalytic.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Procathepsin K, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
Procathepsin K, H. sapiens Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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