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Availability
Please contact us to check the availability
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Immunogen
Recombinant human coagulation factor XIII A (subunit)
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Specificity
human coagulation factor XIII (alpha subunit)
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Species reactivity
human, other species not tested
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Product presentation
Protein A purified antibody from rabbit serum in 10 mM PBS pH7.3, 0.5% BSA, 0.09% sodium azide
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Product category
Primary Antibody
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Antibody s raised in
Rabbit
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Antibody s isotype
IgG
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Antibody s clone
polyclonal
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Product is supplied as
liquid concentrate
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Recommended concentration for use
10-20 µg/ml
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Protein concentration
250 µg/ml
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Positive control
Placenta, dermatofibroma, capillary haemangioma
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Pretreatment
Formaldehyde-fixed tissues are pre-treated with citrate buffer pH 6 (Art.-No. DE000) or Unmasking fluid G (Art. No. DE007) at 96-100°C .
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Tested applications
IHC(C, P), WB
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Recommended secondary reagent
Anti-rabbit IgG:Biotin conjugate (Art. No. ZU101) in combination with streptavidin HRPO conjugate (Art. No. ZU054) or streptavidin alk. phosphatase (Art. No. ZU051)
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Storage method
Store at 2-8°C
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UniProt number
PGo to UniProt/SwissProtGo to UniProt/SwissProt488 (F13A_HUMAN)
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Litterature
1. GOLDBLUM J. R.; TUTHILL R. J. (1997) CD34 and factor-XIIIa immunoreactivity in dermatofibrosarcoma protuberans and dermatofibroma. The American journal of dermatopathology 1997, vol. 19, no2, pp. 147-153 2. KAHN H.J., FEKETE E., FROM L. (2001) Tenascin differentiates dermatofibroma from dermatofibrosarcoma protuberans : Comparison with CD34 and Factor XIIIa. Human Pathology vol. 32, no1, pp. 50-56 3. Y.Song, F.Sakamoto, M.Ito (2005) Characterization of factor XIIIa+ dendritic cells in dermatofibroma: Immunohistochemical, electron and immunoelectron microscopical observations. Journal of Dermatological Science Volume 39, pp 89-96.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by bio logo. Rabbit antibodies are very stable and can be stored for several days at room temperature. bio logo adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Group
Polyclonals and antibodies
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Latin name
Oryctolagus cuniculus
