UbcH5a, human recombinant (His-tag) (New size)
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Catalog number
4851-10
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Price
Please ask
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Size
10 ug
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Synonyms
UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme
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Alternative_names
UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1
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Description
An essential ubiquitin-conjugating enzyme
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Molecular Weight
17.0 kDa
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Storage Temp
-80°C
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Shipping
Dry ice
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Shelf Life
12 months
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Concentration
1.0 mg/ml
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Appearance
Liquid
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Physical form description
Supplied as a liquid in 20 mM Tris-HCl, pH 8, containing 0.5 mM DTT.
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Background Information
UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Conjugation
histidine
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
UbcH5a, recombinant (His-tag) (New size)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Species
Human, Humans
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Alternative name
UbcH5a, H. sapiens Rec. (histidine-detection labelled) (New size)
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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