Recombinant E. coli Ecotin (C-6His)

Description

Recombinant E.coli Ecotin is produced by our E.coli expression system and the target gene encoding Met1-Arg162 is expressed with a 6His tag at the C-terminus.
Species reactivity

E.coli
Origin

Escherichia coli
Peptide sequence

MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVRLEHHHHHH
Estimated molecular weight

19,2 kDa
Protein purity

Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin level

Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Shipping condition

Ambient/Room Temperature
Package form

Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl, 300mM NaCl, pH 8.5.
Storage conditions

Lyophilized protein should be stored at below -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at below -20°C for 3 months.
Reconstitution conditions

See included datasheet or contact us for more information.
UniProt number

P23827
Other related products
Source

Recombinants or rec. proteins
Group

recombinants

Gene target

Ecotin C-6His
Short name

Recombinant Ecotin (C-6His)
Technique

Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. novo supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Species

E. coli, E. coli
Alternative name

E.coli Ecotin(C-6His)
Alternative technique

rec, escherichia

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data