CD5, Clone: CVS5, Mouse Monoclonal antibody-Horse; frozen, IH/flow

  • Catalog number
    YSRTMCA1079
  • Price
    Please ask
  • Size
    2 ml
  • Alternative name 1
    CD5, Clone: CVS5, Mab anti-Horse; frozen, IH/flow
  • Alternative name 2
    CD5, Clone: CVS5, Mab antibody-Horse; frozen, IH/flow
  • Alternative name 3
    CD5, Clone: CVS5, Mab antibody-Horse; frozen, IH/flow
  • Alternative name 4
    anti-CD5 Clone: CVS5 Horse
  • Alternative name 5
    CD5 Clone: CVS5 Horse
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
  • Test
    Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
  • Latin name
    Mus musculus
  • French translation
    anticorps
  • Gene target
    CD5   CVS5   Monoclonal   frozen  
  • Gene symbol
    CD5
  • Short name
    CD5, Clone: CVS5, Mouse Monoclonal antibody- ; frozen, IH/
  • Technique
    Mouse monoclonal, Antibody, frozen, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Species
    Horse, Horses, Mouses
  • Alternative name
    CD5 molecule, clonality: CVS5, Mouse monoclonal (antibody to-)-Horse; -25°C frozen, IH/flow
  • Alternative technique
    antibodies, murine
  • Alternative to gene target
    CD5 molecule, LEU1 and T1, CD5 and IDBG-49730 and ENSG00000110448 and 921, protein binding, Cell surfaces, Cd5 and IDBG-144910 and ENSMUSG00000024669 and 12507, CD5 and IDBG-643103 and ENSBTAG00000013730 and 280745
  • Tissue
    frozen
Gene info
  • Identity
  • Gene
    CD5
  • Long gene name
    CD5 molecule
  • Synonyms gene
  • Synonyms gene name
    • CD5 antigen (p56-62)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
    921
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • CD molecules
    • Scavenger receptor cysteine rich domain containing
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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