Human CellExp™ Kallikrein-11, human recombinant

  • Catalog number
    7417-10
  • Price
    Please ask
  • Size
    10 ug
  • Synonyms
    KLK11, Kallikrein-11, Hippostasin, hK11, PRSS20, TLSP
  • Alternative_names
    KLK11, Kallikrein-11, Hippostasin, hK11, PRSS20, TLSP
  • Description
    May serve as novel biomarkers for ovarian and prostate cancer and other diseases
  • Recombinant
    Yes
  • Source
    HEK293 cells
  • Purity by SDS PAGE
    ≥95%
  • Assay
    SDS-PAGE
  • Biological activity
    Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5’ Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999). J. Biol. Chem. 274, 30468. The specific activity is > 300 pmoles / min / µg.
  • Results
    Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5,5’ Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999). J. Biol. Chem. 274, 30468. The specific activity is > 300 pmoles / min / µg.
  • Molecular Weight
    This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 26.5 kDa. The predicted N-terminus is Glu 19. DTT-reduced Protein migrates as 35-45 kDa due to glycosylation.
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized
  • Physical form description
    Lyophilized from 0.22 um filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
  • Reconstitution Instructions
    Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
  • Background Information
    Kallikrein 11 (KLK11) is also known as Hippostasin, Serine protease 20, Trypsin-like protease, PRSS20, TLSP, which belongs to the peptidase S1 family and Kallikrein subfamily. KLK11 contains one peptidase S1 domain. KLK11 is expressed in brain, skin and prostate. Isoform 1 is expressed preferentially in brain. Isoform 2 is expressed in prostate. Present in seminal plasma at concentrations ranging from 2 to 37 µg/mL (at protein level). Kallikrein 11 /KLK11 is a possible multifunctional protease and efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. KLK11 cleaves synthetic peptides after arginine but not lysine residues.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    CellExp™ Kallikrein-11, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Humans
  • Alternative name
    H. sapiens CellExp™ Kallikrein-11, H. sapiens Rec.
  • Alternative technique
    rec
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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