CD11b/c, Macrophage, Clone: OX-42, Mouse Monoclonal antibody-Rat, FITC
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Catalog numberACL042F-5
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PricePlease ask
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Size0.5mg
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Alternative name 1CD11b/c, Macrophage, Clone: OX-42, Mab anti-Rat, FITC
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Alternative name 2CD11b/c, Macrophage, Clone: OX-42, Mab antibody-Rat, FITC
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Alternative name 3CD11b/c, Macrophage, Clone: OX-42, Mab antibody-Rattus norvegicustus norvegicus, FITC
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Alternative name 4anti-CD11b/c Macrophage Clone: OX-42
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Alternative name 5CD11b/c Macrophage Clone: OX-42
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolSNORD115-42, IGLVI-42, HCRT, IGKV1D-42, IGHV3-42, RNU6-42P, CD200, TNFSF4, DSCAM, NFX1
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Short nameCD11b/c, Macrophage, Clone: OX-42, Mouse Monoclonal antibody- , FITC
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TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelFITC
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SpeciesRat, Mouses, Rats
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Alternative nameCD11b/c, Macrophage, clonality: OX-42, Mouse monoclonal (antibody to-)-Rat, fluorecein
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Alternative techniqueantibodies, murine, fluorescine
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Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, C/D box 115-42
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-02-15
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, C/D box
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin lambda variable (I)-42 (pseudogene)
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Synonyms gene name
- immunoglobulin lambda variable (I)-42
- immunoglobulin lambda variable (I)-42 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-08
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin lambda locus at 22q11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namehypocretin neuropeptide precursor
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Synonyms gene name
- hypocretin (orexin) neuropeptide precursor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-04-21
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1D-42 (non-functional)
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Synonyms gene name
- immunoglobulin kappa variable 1D-42
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin kappa locus at 2p11.2
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable 3-42 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable 3-42
- immunoglobulin heavy variable 3-42 pseudogene
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GenBank acession
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Locus
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Discovery year2000-04-04
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRNA, U6 small nuclear 42, pseudogene
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Synonyms gene
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Synonyms gene name
- RNA, U6 small nuclear 42
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Locus
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Discovery year2008-06-10
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameCD200 molecule
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Synonyms gene
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Synonyms gene name
- antigen identified by monoclonal antibody MRC OX-2
- CD200 antigen
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Synonyms
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTNF superfamily member 4
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Synonyms gene
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Synonyms gene name
- tax-transcriptionally activated glycoprotein 1, 34kD
- tumor necrosis factor (ligand) superfamily, member 4
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-15
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Entrez gene record
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Pubmed identfication
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Classification
- Tumor necrosis factor superfamily
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameDS cell adhesion molecule
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Synonyms gene name
- Down syndrome cell adhesion molecule
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ig-like cell adhesion molecule family
- I-set domain containing
- Fibronectin type III domain containing
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear transcription factor, X-box binding 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-05-26
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Pubmed identfication
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Classification
- PHD finger proteins
- Ring finger proteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data