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Long name
Mouse Monoclonal Antibody against Human CD146, Clone: P1H12
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Antigen
CD146
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Canine (Dog), Rabbit
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Negative species reactivity
Rat
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Clonality
Monoclonal Antibody (Mab)
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Clone
P1H12
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Applications
FC, IP, WB, IHC(P), IHC(F), ICC
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Immunogen
cultured human umbilical cells
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Format conjugation
PE
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Specificity
The mouse monoclonal antibody P1H12 recognizes CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes. _x000D_ _x000D_ Workshop: HLDA 8
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Research area
CD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD146, Clone: P1H12 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50% of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Finney MR, Greco NJ, Haynesworth SE, Martin JM, Hedrick DP, Swan JZ, Winter DG, Kadereit S, Joseph ME, Fu P, Pompili VJ, Laughlin MJ: Direct comparison of umbilical cord blood versus bone marrow-derived endothelial precursor cells in mediating neovascularization in response to vascular ischemia. Biol Blood Marrow Transplant. 2006 May;12(5):585-93., *Solovey A, Lin Y, Browne P, Choong S, Wayner E, Hebbel RP: Circulating activated endothelial cells in sickle cell anemia. N Engl J Med. 1997 Nov 27;337(22):1584-90., *Kamstock D, Guth A, Elmslie R, Kurzman I, Liggitt D, Coro L, Fairman J, Dow S: Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma. Cancer Gene Ther. 2006 Mar;13(3):306-17., *Solovey AN, Gui L, Chang L, Enenstein J, Browne PV, Hebbel RP: Identification and functional assessment of endothelial P1H12. J Lab Clin Med. 2001 Nov;138(5):322-31.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
