Saturday, July 28, 2007
For cells, cell lines and tissues in culture till half confluency.
The gelatin protein mixture of Trevigen is called Cultrex and it is a matrigel like secreted supernatant of the EHS mouse sarcoma cell line. It is a 3D structure similar to Tissues and cells will to adhere to this basement membrane extract BME very well. Cultrexes are the best medium for 3D cell culture studies. They can be used as a metastasis model or an iPSC model (induced pluripotent stem cell). The cultrex medium is useful for cell migration studies using the Pathclear endotoxin free and sterile basement membrane extract. Enghelbreth Holm Swarm sarcoma cells are sold by Corning. A collagen, laminin, fibronectin and vitrobectin Cultrexes are available from Trevigen. Cultrex® Human has been tested for the viruses should be handled in a BSL2 ( biosafety level 2 ) environment.Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Cultrex Stem Qualified Fibronectin, PathClear
Cultrex progenitor cellular Qualified H. sapiens Fibronectin, PathClear