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Gene info
Clusterin (SGP-2, SP-40,40, pADHC-9, TRPM-2, CLJ, T64, GP III, APO J, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in several physiological processes. Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has a number of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress-induced insults caused by degraded and misfolded protein precipitates. The protein is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin has not been defined.
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Gene names
CLU, APO-J, Apolipoprotein J, CLU1, Clusterin, Complement lysis inhibitor, CLI, AAG4, Aging-associated protein 4, Ku70-binding protein 1, KUB1, SGP2, APOJ, SGP-2, Sulfated glycoprotein 2, CLU2, Complement cytolysis inhibitor, NA1/NA2, SP-40
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Antibodie s label
unlabelled serum
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Purity
Protein A Column
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Ab raised in
Mouse
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Type
Monoclonal
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Monoclonal
[Hs-3]
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Immunoglobulin
IgG1
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Antigen
Clusterin monoclonal antibody was made against freshly ejaculated human sperms washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization (Human).
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Immuno application
ELISA, immuno-histo-chemistry, immuno-cyto-chemistry
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Antibody advice
Clusterin antibody can be used in ELISA, and immunohistochemistry starting at 20 ug/mL.
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Suspention pH azide
PBS, 15 mM sodium azide, approx., pH 7.4.
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Keep at
Clusterin antibody can be stored short term 4 grades C. For long term storage aliquot and store at -20 grades C. As with all antibodies avoid freeze/thaw cycles.
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Works with
Human
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Omim nr
116533
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Ncbi nr
P10909
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Vial with antibody
in solution
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NCBI GENE nr
1191
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GENE symbol
CLU
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Antigene name
clusterin
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Source
Homo sapiens
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Protein nr
P10909
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
