Anti-NCF1/p47 phox(Ser359), ALEXA Fluor 594

• Catalog number
  GENTObs-11445R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  NCF1/p47 phox Ser359
• Specificity
  This antibody reacts specifically with NCF1/p47 phox(Ser359)
• Modification
  Phosphorylation
• Modification site
  Ser359
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  N/A
• Antigen Source
  KLH conjugated synthetic phosphopeptide derived from human NCF1 around the phosphorylation site of Ser359
• Gene ID
  653361
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  The heredity chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67-phox has been shown to interact with the proline-rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  NCF1Ser359; p47 phoxSer359; NCF1 phospho S359; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; NOXO2; p47 phox; p47phox; ;SH3 and PX domain containing protein 1A; SH3PXD1A; NADPH oxidase p47 phox; NCF1_HUMAN.
• Also known as
  NCF1/p47 phox(Ser359) Antibody
• Other name
  Anti-NCF1/p47 phox(Ser359)
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.

• Gene target
  NCF1/p47   phox   Ser359  
• Gene symbol
  NCF1
• Short name
  Anti-NCF1/p47 phox(Ser359)
• Technique
  anti-, anti, antibody to, antibodies
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  NCF1/p47 phox(Ser359) Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  antibodies
• Alternative to gene target
  neutrophil cytosolic factor 1, NCF1 and IDBG-21570 and ENSG00000158517 and 653361, phosphatidylinositol-3, Plasma membranes, Ncf1 and IDBG-202592 and ENSMUSG00000015950 and 17969, NCF1 and IDBG-639883 and ENSBTAG00000003305 and 281345

Gene info

• Identity
  HGNC:7660
• Gene
  NCF1
• Long gene name
  neutrophil cytosolic factor 1
• Synonyms gene name
  • neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1)
• Synonyms
  • p47phox
  • NOXO2
  • NCF1A
  • SH3PXD1A
• Synonyms name
  • NADPH oxidase organizer 2
  • chronic granulomatous disease, autosomal 1
• GenBank acession
  • M25665
• Locus
  7q11.23
• Discovery year
  1989-05-25
• Entrez gene record
  653361
• RefSeq identity
  • NM_000265
• VEGA ID
  OTTHUMG00000149965
• Locus Specific Databases
  • NCF1base: Mutation registry for autosomal recessive chronic granulomatous disease (CGD), deficiency of p47phox
  • LRG_87

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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