SMAD4 Antibody

  • Catalog number
    R31056
  • Price
    Please ask
  • Size
    0.1mg
  • Category
    Antibody
  • Concentration
    0.5mg/ml if reconstituted with 0.2ml sterile DI water
  • Form
    Antigen affinity purified
  • Conjugation
    Unconjugated
  • Clone
    Polyclonal antibody
  • Recognised antigen
    SMAD4
  • Host animal
    Rabbit (Oryctolagus cuniculus)
  • Clonality
    Polyclonal (rabbit origin)
  • Species reactivity
    Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
  • Tested applications
    WB, IHC-P
  • Recommended dilutions
    Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
  • Notes
    The stated application concentrations are suggested starting amounts. Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
  • Intented use
    This SMAD4 antibodyis to be used only for research purposes and not for diagnostics..
  • Uniprot
    Q13485
  • Purity
    Antigen affinity
  • Description
    Mothers Against Decapentaplegic Drosophila Homolog of 4, also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFbeta protein superfamily. Hahn et al.(1996) identified the gene on chromosome 18q21.1. Howe et al.(1998) identified the gene within a region on 18q21.1 defined by linkage analysis in a kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.
  • Immunogen
    An amino acid sequence from the N-terminus of human SMAD4 (RLWRWPDLHKNELKHVK) was used as the immunogen for this SMAD4 antibody (100% homologous in human, mouse and rat).
  • Storage
    After reconstitution, the Smad4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
  • Properties
    If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
  • Gene target
    SMAD4  
  • Gene symbol
    SMAD4
  • Short name
    Anti-SMAD4
  • Technique
    Antibody, antibodies against human proteins, antibodies for
  • Isotype
    Rabbit IgG
  • Alternative name
    Antibodies to SMAD4
  • Alternative technique
    antibodies
  • Alternative to gene target
    SMAD family member 4, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, transforming growth factor beta receptor, nuclei, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248
Gene info
  • Identity
  • Gene
  • Long gene name
    SMAD family member 4
  • Synonyms gene
  • Synonyms gene name
    • MAD, mothers against decapentaplegic homolog 4 (Drosophila)
    • SMAD, mothers against DPP homolog 4 (Drosophila)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1996-11-15
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • SMAD family
  • VEGA ID
  • Locus Specific Databases
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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