EZExtract™ Polyubiquitin Buffer Kit

• Catalog number
  K6570-3
• Price
  Please ask
• Size
  3 components

• Description
  Set of buffers and reagents required for the extraction of ubiquitinated or sumoylated proteins. EZExtract™ Polyubiquitin Buffer Kit is ideal for efficient extraction of polyubiquitin modified proteins from cells. Ubiquitin is a highly conserved 76-amino acid protein. It can be conjugated via its C-terminus to the amine groups of lysine residues on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. Different types of polyubiquitin chains can form, depending on the internal lysine residue used for this conjugation. These polyubiquitin chains further can attach to proteins post-translationally and aid in numerous downstream activities like proteasome-mediated proteolysis, autophagy, DNA damage tolerance, inflammation, apoptosis, signal transduction etc. Several classes of Ubiquitin interacting proteins help in mediating these downstream effects. EZExtract™ Polyubiquitin Buffer kit efficiently extracts polyubiquitinated proteins from cells while preserving the ubiquitination state of protein samples.
• Product Highlights
  Key Features: • Highly efficient in lysing cells. • Preserves ubiquitination or sumoylation status during downstream processing. • Compatible with mass spectrometry. Applications: • Extraction of cells/tissues to achieve optimal yield of ubiquitinated or sumoylated proteins. • General lysis buffer for a broad range of applications, including Western blot, ELISA, dot blot, enzymatic assays, etc.
• Storage Temp
  MT
• Shipping
  gel pack
• Shelf Life
  12 months
• Datasheet
• Notes
  For research use only. Not to be used for human or animal treatment or consumption.
• Additional description
  Buffering solutions are useful to keep the pH range sable when using this reagent of Biovision.

• Gene target
  EZExtract™   Polyubiquitin   Kit  
• Short name
  EZExtract™ Polyubiquitin Kit
• Alternative name
  EZExtract™ Polyubiquitin solution reagent
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products