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Type
Conjugated Primary Antibody
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Conjugated with
ALEXA FLUOR® 594
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
CDKN2D
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Specificity
This antibody reacts specifically with CDKN2D
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
N/A
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Antigen Source
KLH conjugated synthetic peptide derived from human CDKN2D/p19 INK4d
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Gene ID
1032
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Swiss Prot
N/A
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
The protein encoded by this gene is a member of the INK4 family of cyclin-dependent kinase inhibitors. This protein has been shown to form a stable complex with CDK4 or CDK6, and prevent the activation of the CDK kinases, thus function as a cell growth regulator that controls cell cycle G1 progression. The abundance of the transcript of this gene was found to oscillate in a cell-cycle dependent manner with the lowest expression at mid G1 and a maximal expression during S phase. The negative regulation of the cell cycle involved in this protein was shown to participate in repressing neuronal proliferation, as well as spermatogenesis. Two alternatively spliced variants of this gene, which encode an identical protein, have been reported. [provided by RefSeq, Jul 2008].
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
590nm/617nm
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Synonyms
CDK inhibitor p19INK4d; CDKN2D; cell cycle inhibitor, Nur77 associating protein; Cyclin dependent kinase inhibitor 2D; Cyclin-dependent kinase 4 inhibitor D; Cyclin-dependent kinase inhibitor 2D p19, inhibits CDK4; inhibitor of cyclin-dependent kinase 4d; INK4D; p19; p19-INK4D; p19INK4d; Similar to cyclin-dependent kinase inhibitor 2D; CDN2D_HUMAN.
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Also known as
CDKN2D Polyclonal Antibody
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Other name
Anti-CDKN2D Polyclonal
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
For facs or microscopy Alexa 1 conjugate.
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Conjugation
Alexa Fluor, ALEXA FLUOR® 594
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Conjugated
Alexa conjugate 1
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
CDKN2D
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Short name
Anti-CDKN2D
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
ALEXA FLUOR 594
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Alternative name
CDKN2D Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4), INK4D and p19 and p19-INK4D, CDKN2D and IDBG-27803 and ENSG00000129355 and 1032, protein kinase binding, nuclei, Cdkn2d and IDBG-738755 and ENSMUSG00000096472 and 12581, CDKN2D and IDBG-643316 and ENSBTAG00000010731 and 510244
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