Human CellExp™ VEGF 121, Human Recombinant

• Catalog number
  6484-10
• Price
  Please ask
• Size
  10 µg

• Synonyms
  Human Cellexp Human Recombinant VEGF 121
• Alternative_names
  Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
• Description
  A signaling protein involved in both vasculogenesis and angiogenesis
• Recombinant
  Yes
• Source
  Human 293 cell expressed
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 ug of protein
• Biological activity
  ED₅₀ is typically 2 to 8 ng/mL. The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (Human Umbilical Vein Endothelial Cells).
• Results
  2 to 8 ng/ml
• Molecular Weight
  37 kDa, homodimer; 50 kDa, homotrimer, glycosylated
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized in PBS.
• Reconstitution Instructions
  Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.
• Background Information
  VEGF is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophage migration, neurons, cancer cells, kidney epithelial cells).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   VEGF   121  
• Gene symbol
  VEGFA, VEGFD, VEGFC
• Short name
  CellExp™ VEGF 121, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ VEGF 121, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:12680
• Gene
  VEGFA
• Long gene name
  vascular endothelial growth factor A
• Synonyms gene
  • VEGF
• Synonyms gene name
  • vascular endothelial growth factor
• Synonyms
  • VEGF-A
  • VPF
• GenBank acession
  • AB021221
• Locus
  6p21.1
• Discovery year
  1994-01-10
• Entrez gene record
  7422
• Pubmed identfication
  • 8786112
• RefSeq identity
  • NM_001025366
• Classification
  • VEGF family
• VEGA ID
  OTTHUMG00000014745
• Locus Specific Databases
  • ALSOD, the Amyotrophic Lateral Sclerosis Online Genetic Database

Gene info

• Identity
  HGNC:3708
• Gene
  VEGFD
• Long gene name
  vascular endothelial growth factor D
• Synonyms gene
  • FIGF
• Synonyms gene name
  • c-fos induced growth factor (vascular endothelial growth factor D)
• Synonyms
  • VEGF-D
• GenBank acession
  • AJ000185
• Locus
  Xp22.2
• Discovery year
  1997-03-27
• Entrez gene record
  2277
• Pubmed identfication
  • 9479493
• RefSeq identity
  • NM_004469
• Classification
  • VEGF family
• VEGA ID
  OTTHUMG00000021175
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:12682
• Gene
  VEGFC
• Long gene name
  vascular endothelial growth factor C
• Synonyms
  • VRP
  • VEGF-C
• Synonyms name
  • vascular endothelial growth factor-related protein
• GenBank acession
  • BC035212
• Locus
  4q34.3
• Discovery year
  1996-10-26
• Entrez gene record
  7424
• Pubmed identfication
  • 8617204
• RefSeq identity
  • NM_005429
• Classification
  • VEGF family
• VEGA ID
  OTTHUMG00000160734

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products