Rabbit Anti-Human MAK (pThr157) Polyclonal: RPE
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Catalog numberSPC-1013D-RPE
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PricePlease ask
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Size100 µl
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ImmunogenA phospho-specific peptide corresponding to residues surrounding Thr157 of human MAK (AA154-160)
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Antibody s targetHuman MAK (pThr157)
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Antibody s full descriptionRabbit Anti-Human MAK (pThr157) Polyclonal
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Antibody s categoryPolyclonal Antibodies
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Antibody s other nameIck antibody, Mak antibody, MAK_HUMAN antibody, Male germ cell associated kinase antibody, Rck antibody, RP62 antibody, Serine/threonine protein kinase MAK antibody, Serine/threonine-protein kinase MAK antibody
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Verified applicationsWB, AM
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Raised inRabbit
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Antibody s reactivityHuman
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Antibody s recommended dilutions for useWB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified fromPeptide Affinity Purified
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Recommended buffer for storagePBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentrationSee included datasheet or contact our support service
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Antibody s specificityDetects 70.581 kDa.
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Storage recommendations-20°C
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Shipping recommendationsBlue Ice or 4°C
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Certificate of analysisA 1:250 dilution of SPC-1013 was sufficient for detection of MAK (pThr157) in 10 µg of HepG2 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cellNucleus, Cytoplasm, Cytoskeleton, Centrosome
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Tissue specificityExpressed in prostate cancer cell lines at generally higher levels than in normal prostate epithelial cell lines. Isoform 1 is expressed in kidney, testis, lung, trachea, and retina. Isoform 2 is retina-specific where it is expressed in rod and cone photoreceptors.
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Scientific contextSee included datasheet or contact our support service.
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BibliographyRefer to PubMed
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Released date29/Aug/2016
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NCBI numberNP_005897
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Gene number4117
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Protein numberP20794
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PubMed numberRefer to PubMed
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Tested applicationsTo be tested
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Tested reactivityTo be tested
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Antibody s datasheetContact our support service to receive datasheet or other technical documentation.
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Representative figure linkNo Data Available
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Warning informationNon-hazardous
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Country of productionCanada
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Total weight kg1.4
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Net weight g0.1
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Stock availabilitIn Stock
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestStressMark antibodies supplies antibodies that are for research of human proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Gene target
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Gene symbolRPE, MAK, ALPK3
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Short nameRabbit Anti- MAK (pThr157) Polyclonal: RPE
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Techniqueanti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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HostRabbit, Rabbits
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IsotypeImmunoglobulin
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LabelRPE
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SpeciesHuman, Humans
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Alternative nameproduction species: rabbit Antibody toHuman MAK (pThr157) polyclonal: RPE
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Alternative techniquepolyclonals, rabbit-anti, antibodies
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Gene info
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Identity
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Gene
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Long gene nameribulose-5-phosphate-3-epimerase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemale germ cell associated kinase
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Synonyms gene name
- male germ cell-associated kinase
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Synonyms
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Locus
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Discovery year1994-02-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namealpha kinase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-12-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- I-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data