Target of this antibody:
Alternative names of antibody target:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, three families of mammalian DNA methyltransferase genes have been identified which include DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The DNMT3 family members, DNMT3a and DNMT3b, are strongly expressed in embryonic stem (ES) cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of DNMT3b gene occur in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
Clonality of antibody:
This antibody reacts with:
Recombinant mouse Dnmt3a expressed in bacteria. The epitope was found to be located near the C-terminus (amino acids 705-908).
Formulation of this antibody:
50 µg of antibody in 100 µl PBS containing 0.05% BSA and 0.05% sodium azide.
Protein G purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, Immunohistochemistry
Western blot: 2 µg/ml, ICC/IF: 5 µg/ml, ChIP/IHC (paraffin embedded sections). However, the optimal conditions should be determined individually.
anti-DNA Methyltransferase 3a (Clone 64B1446) Antibody
antibody to-Desoxyribonucleic acid Methyltransferase 3a (clonality 64B1446) (Antibody to)
Long gene name protein kinase, DNA-activated, catalytic subunit
Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
- DNA-dependent protein kinase catalytic subunit
Discovery year 1993-11-09
Entrez gene record 5591
- Armadillo like helical domain containing
Havana BLAST/BLAT OTTHUMG00000164239