UbcH1, human recombinant (GST-tag)

• Catalog number
  4846-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
• Alternative_names
  UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
• Description
  An essential ubiquitin-conjugating enzyme
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Molecular Weight
  22.4 kDa
• Storage Temp
  -80°C
• Shipping
  Dry ice
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT.
• Background Information
  Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  UbcH1   GST-tag  
• Gene symbol
  MGST3
• Short name
  UbcH1, recombinant (GST-tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  tag
• Species
  Human, Humans
• Alternative name
  UbcH1, H. sapiens Rec. (GST-detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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