Rabbit Anti-Human PCNA Polyclonal: PerCP

  • Catalog number
    SPC-691D-PCP
  • Price
    Please ask
  • Size
    100 µg
  • Immunogen
    Synthetic peptide of Human PCNA
  • Antibody s target
    Human PCNA
  • Antibody s full description
    Rabbit Anti-Human PCNA Polyclonal
  • Primary research fields
    Cancer, Tumor Biomarkers, Tumor Antigens, Cell Signaling, Epigenetics and Nuclear Signalling, DNA/RNA, DNA Synthesis
  • Antibody s category
    Polyclonal Antibodies
  • Antibody s other name
    cb16 Antibody, MGC8367 Antibody, PCNA_HUMAN Antibody, Polymerase delta accessory protein Antibody, DNA polymerase delta auxiliary protein Antibody, Proliferating cell nuclear antigen Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, PCNAR Antibody, Pcna/cyclin Antibody, HGCN8729 Antibody, wu:fb36g03 Antibody, PCNA Antibody, ATLD2 Antibody, fb36g03 Antibody, OTTHUMP00000030190 Antibody, Cyclin Antibody
  • Verified applications
    WB
  • Raised in
    Rabbit
  • Antibody s reactivity
    Human
  • Antibody s recommended dilutions for use
    WB (1:1000); optimal dilutions for assays should be determined by the user.
  • Antibody s purified from
    Peptide Affinity Purified
  • Recommended buffer for storage
    PBS, 50% glycerol, 0.09% sodium azide
  • Antibody s concentration
    1 mg/ml
  • Antibody s specificity
    Detects ~37 kDa.
  • Storage recommendations
    -20°C
  • Shipping recommendations
    Blue Ice or 4°C
  • Certificate of analysis
    A 1:1000 dilution of SPC-691 was sufficient for detection of PCNA in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
  • Antibody in cell
    Nucleus
  • Scientific context
    See included datasheet or contact our support service.
  • Bibliography
    Refer to PubMed
  • Released date
    29/Aug/2016
  • NCBI number
    NP_002583.1
  • Gene number
    5111
  • Protein number
    P12004
  • PubMed number
    Refer to PubMed
  • Tested applications
    To be tested
  • Tested reactivity
    To be tested
  • Antibody s datasheet
    Contact our support service to receive datasheet or other technical documentation.
  • Representative figure legend
    Western blot analysis of Human 293 Trap cell lysates showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa. Rabbit Anti-PCNA Antibody used in Western blot (WB) on 293 Trap cell lysates (SPC-691)
  • Warning information
    Non-hazardous
  • Country of production
    Canada
  • Total weight kg
    1.4
  • Net weight g
    0.1
  • Stock availabilit
    In Stock
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    StressMark antibodies supplies antibodies that are for research of human proteins.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugate
    Peridinin chlorophyll (PerCP)
  • Group
    Polyclonals and antibodies
  • About
    Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    PCNA  
  • Gene symbol
    PCNA, PCNA-AS1
  • Short name
    Rabbit Anti- PCNA Polyclonal: PerCP
  • Technique
    anti-Human, Polyclonal, Rabbit, anti-, Percp, anti, antibody to, antibodies, antibodies against human proteins, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the Rabbit Anti- PCNA Polyclonal: PerCP is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from StressMark antibodies. Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
  • Host
    Rabbit, Rabbits
  • Isotype
    Immunoglobulin
  • Label
    PerCP
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit Antibody toHuman PCNA polyclonal: PerCP
  • Alternative technique
    polyclonals, rabbit-anti, antibodies-percp, antibodies
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    PCNA antisense RNA 1
  • Synonyms gene
  • Synonyms gene name
    • PCNA antisense RNA (non-protein coding)
    • PCNA antisense RNA 1 (non-protein coding)
  • Locus
  • Discovery year
    2009-08-18
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Antisense RNAs
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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