GOT1, human recombinant

• Catalog number
  7808-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18
• Alternative_names
  Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18
• Description
  Plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is > 13 unit/mg, in which one unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
• Results
  Specific activity is > 13 unit/mg
• Unit Definition
  One unit will convert 1.0 µmole of alpha-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25ºC.
• Molecular Weight
  48.4 kDa (433 aa, 1-413 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 100 mM NaCl and 1 mM DTT.
• Background Information
  Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GOT1  
• Gene symbol
  GOT1-DT, GOT1, GOLT1B, GOLT1A
• Short name
  GOT1, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  GOT1, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:55848
• Gene
  GOT1-DT
• Long gene name
  GOT1 divergent transcript
• Locus
  10q24.2
• Discovery year
  2021-09-01
• Entrez gene record
  101927300
• Classification
  • Divergent transcripts

Gene info

• Identity
  HGNC:4432
• Gene
  GOT1
• Long gene name
  glutamic-oxaloacetic transaminase 1
• Synonyms gene name
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • glutamic-oxaloacetic transaminase 1, soluble
• Synonyms
  • AST1
• Synonyms name
  • aspartate aminotransferase 1
  • aspartate transaminase 1
  • glutamate oxaloacetate transaminase 1
• GenBank acession
  • M37400
• Locus
  10q24.2
• Discovery year
  2001-06-22
• Entrez gene record
  2805
• Pubmed identfication
  • 1974457
• RefSeq identity
  • NM_002079
• VEGA ID
  OTTHUMG00000018882

Gene info

• Identity
  HGNC:20175
• Gene
  GOLT1B
• Long gene name
  golgi transport 1B
• Synonyms gene name
  • golgi transport 1 homolog B (S. cerevisiae)
• Synonyms
  • CGI-141
  • YMR292W
  • GOT1
• GenBank acession
  • AB097020
• Locus
  12p12.1
• Discovery year
  2005-02-07
• Entrez gene record
  51026
• Pubmed identfication
  • 12414650
  • 10810093
• RefSeq identity
  • NM_016072
• VEGA ID
  OTTHUMG00000169133

Gene info

• Identity
  HGNC:24766
• Gene
  GOLT1A
• Long gene name
  golgi transport 1A
• Synonyms gene name
  • golgi transport 1 homolog A (S. cerevisiae)
• Synonyms
  • FLJ42654
  • CGI-141
  • YMR292W
  • GOT1
  • MGC62027
• GenBank acession
  • BC058832
• Locus
  1q32.1
• Discovery year
  2005-02-07
• Entrez gene record
  127845
• Pubmed identfication
  • 26255816
• RefSeq identity
  • NM_198447
• VEGA ID
  OTTHUMG00000036056

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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