NZYM Growth Agar is used for lambda and filamentous phage. To prepre the media d issolve the powder in d istilled water, heat to boiling if it is necessary, sterilize by autoclaving at 121°C for 15 minutes, then mix well and d ispense as desired.
Appearance of powder
Cream to yellow coloured, homogeneous, free flowing powder.
Quantity required by litre
Organisms (ATCC) Growth
Escherichia coli: good-luxuriant
Colour and Clarity
Light yellow coloured, clear to slightly opalescent gel forms in Petri plates.
Casein enzymic hydrolysate 10.00 Yeast extract 5.00 MgSO4. 7H2O 2.00 Sodium chloride 5.00 Agar 15.00 Final pH (at 25oC) 7.0 ± 0.2
Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours.
Store below 30°C and the prepared medium at 2 - 8°C.
Molecular Biology Growth Media for Escherichia coli