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Availability
Please contact us to check the availability
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Immunogen
Recombinant protein, soluble receptor human RAGE
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Specificity
Human AGE receptor
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Species reactivity
Human, other species not determined
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Product presentation
Protein G affinity purified IgG in PBS containing 2% Block Ace as stabilizer and 0.1% Proclin as bacteriostat
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1,k
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Antibody s clone
1C5
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Product is supplied as
liquid concentrate
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Recommended concentration for use
0.1 µg/ml WB, 1 µg/ml ICC, 1 µg/ml FACS, 0.01 µg/ml ELISA
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Protein concentration
250 µg/ml
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Positive control
Diabetic kidney, arteriosclerosis
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Tested applications
IHC, WB
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Recommended secondary reagent
Recommended secondary systems in IHC are our Universal Staining Kits Ref. AE005 or DA005 or the VECTASTAIN Elite ABC System Mouse IgG (Ref. PK-6102). The latter is also suitable for Western Blotting or other techniques.
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Storage method
Store at -20°C
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UniProt number
Q151Go to UniProt/SwissProt9 (RAGE_HUMAN)
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Litterature
1. Neeper M, Schmidt AM,Brett J,et al.:Cloning and expression of a cell surface receptor for advanced glycosylation end products of protein.J Biol Chem 267:14998-15004,1992 2. Schmidt AM ,Viannaet M ,Gerlach M,et:al:Isolation and characterization of two binding proteins for advanced glycosylation end products from bovine lung which are present on the endothelial cell surface. J Biol Chem 267:14987-14997,1992
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Tips
Gentaur antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
