Mouse Monoclonal IgG1/k Anti-GATA3 (L50-823) [L50-823]

• Catalog number
  GAT001-G
• Price
  Please ask
• Size
  5 ml

• Availability
  Please contact us to check the availability
• Immunogen
  Conserved peptide between the GATA trans-activation and DNA-binding domain
• Specificity
  GATA3; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3
• Species reactivity
  Human, rat, others not tested
• Product presentation
  Purified antibody in PBS with BSA and 15 mM sodium azide
• Product category
  Primary Antibody
• Antibody s raised in
  Mouse
• Antibody s isotype
  IgG1/k
• Antibody s clone
  L50-823
• Product is supplied as
  ready to use
• Recommended concentration for use
  neat
• Protein concentration
  N/A
• Positive control
  Breast carcinoma, Urothelial carcinoma (nuclear staining)
• Pretreatment
  HIER (heat induced epitope retrieval) using BioLogo's Unmasking Fluid T (TEC-Buffer, pH 8, Art. No. DE005).
• Tested applications
  IHC (P)
• Recommended secondary reagent
  We recommend the use of our Universal Staining Systems AEC or DAB (Art. No. AE005 and DA005) as well as ABC- or Polymer Systems containing anti-mouse IgG conjugates.
• Storage method
  Store at 2-8°C
• UniProt number
  P23771 (GATA3_HUMAN)
• Litterature
  1. Higgins JP et al. Placental S100 (S100P) and GATA3: markers for transitional epithelium and urothelial carcinoma discovered by complementary DNA mircoarray. Am J Surg Pathol. 2007; 31:673-680. 2. Liu H et al. Immunohistochemical evaluation of GATA3 expression in tumors and normal tissues. Am J Clin Pathol. 2012; 138:57-64. 3. Miettinen M et al. GATA 3 – A multispecific but potentially useful marker in surgical pathology – a systematic analysis of 2500 epithelial and non-epithelial tumors. Am J Surg Pathol. 2014; 38:13-22.
• Tips
  *Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
• Other products
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  Monoclonal   IgG1/k   GATA3   L50-823   [L50-823]  
• Gene symbol
  MRPL50, GATA3, GATA3-AS1
• Short name
  Research
• Technique
  Mouse monoclonal, anti-, Mouse, anti, antibody to, antibodies, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Isotype
  IgG1
• Species
  Mouse, Mouses
• Alternative name
  GATA3 (L50-823)
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:16654
• Gene
  MRPL50
• Long gene name
  mitochondrial ribosomal protein L50
• Synonyms
  • FLJ20493
  • MRP-L50
• Synonyms name
  • mitochondrial 39S ribosomal protein L50
• GenBank acession
  • AK000500
• Locus
  9q31.1
• Discovery year
  2001-10-12
• Entrez gene record
  54534
• RefSeq identity
  • NM_019051
• Classification
  • Mitochondrial ribosomal proteins
  • Large subunit mitochondrial ribosomal proteins
• VEGA ID
  OTTHUMG00000020384

Gene info

• Identity
  HGNC:4172
• Gene
  GATA3
• Long gene name
  GATA binding protein 3
• Synonyms gene name
  • GATA-binding protein 3
• Synonyms
  • HDR
• GenBank acession
  • X55122
• Locus
  10p14
• Discovery year
  1992-11-03
• Entrez gene record
  2625
• Pubmed identfication
  • 2050118
  • 15087456
• RefSeq identity
  • NM_001002295
• Classification
  • GATA zinc finger domain containing
• VEGA ID
  OTTHUMG00000017640

Gene info

• Identity
  HGNC:33786
• Gene
  GATA3-AS1
• Long gene name
  GATA3 antisense RNA 1
• GenBank acession
  • BC036297
  • AK128810
• Locus
  10p14
• Discovery year
  2012-12-04
• Entrez gene record
  399717
• Pubmed identfication
  • 23870669
  • 30420860
• RefSeq identity
  • NR_104327
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000156966

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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