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Availability
Please contact us to check the availability
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Immunogen
Purified human endothelial vesicles
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Specificity
Human CD34 antigen (Epitope neuraminidase resistant , glycoprotease- and chymopapain-sensitive)
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Species reactivity
Human, Rhesus Monkey, no cross-reactivity with cattle, dog, rat and sheep
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Product presentation
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
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Product category
Primary Antibody
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Antibody s raised in
Mouse
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Antibody s isotype
IgG1,k
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Antibody s clone
QBEND/10
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Product is supplied as
liquid concentrate
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Recommended concentration for use
1:10 - 1:20
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Protein concentration
40 µg/ml
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Positive control
Tonsil, bone marrow
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Pretreatment
No protease pre-treatment!, Pre-treatment with unmasking fluid G (Art. No. DE007) or unmasking fluid C (Art. No. DE000) at 90-100°C may be helpful.
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Tested applications
IHC(P)
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Recommended secondary reagent
We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
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Storage method
Store at 2-8°C
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UniProt number
Go to UniProt/SwissProt
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Litterature
1. Civin C.I., Strauss L.C., Brovall C., Fackler M.J. , Schwartz J.F., Shaper J.H. (1984) Antigenic analysis of haematopoesis. III. A hematopoetic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-Ia cells. J. Immunol. 133; 157-165. 2. Civin C.I., Trischmann T.M., Fackler M.J., Bernstein I.D., Bühring H.J., Campos L., et al. (1989) M7.1. Report on the CD34 cluster workshop. In: Kapp W. et al. Eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford, New York, Tokyo: Oxford University Press pp. 818-825. 3. Fina L. et al. (1990) Expression of the CD34 gene in vascular endothelial cells. Blood 75; 2417-2426. 4. Dercksen M.W., Daams G.M., de Haas M., von dem Borne A.E.G.K., and van der Schoot C.E. M10.2. Charakterization of the CD34 cluster. In: Schlossman S.F. et al. Eds. Leucocyte Typing V. White Cell Differentiation Antigens. Oxford, New York, Tokyo: Oxford University Press pp. 850-853.
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Tips
*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
bio logo supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Latin name
Mus musculus
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Gene target
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Gene symbol
CD34
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Short name
Bioprime
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Technique
Mouse monoclonal, anti-Human, anti-, Mouse, anti, antibody to, antibodies, antibodies against human proteins, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Species
Human, Humans, Mouses
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Alternative name
CD34, human
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Alternative technique
murine, antibodies
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Alternative to gene target
CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
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