Rat BAK1(BCL2 Antagonist/Killer 1) EIA Kit
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Catalog number
E-EL-R0097-96T
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Price
Please ask
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Size
96Test
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Detection Type
Colormetric
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Target Species
Rat
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Target Synonym
BAK, BAK-LIKE, BCL2L7, CDN1, Bcl-2 Homologous Antagonist/Killer
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Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat BAK1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BAK1. You can calculate the concentration of Rat BAK1 in the samples by comparing the OD of the samples to the standard curve.
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Sensitivity
0.47ng/mL
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Detection Range
0.78~50ng/mL
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Target Name
BAK1
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UNIProt ID
Q9JK59
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Tested Sample Types
Serum, Plasma, Cell supernatant
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Product Name
Rat BAK1(BCL2 Antagonist/Killer 1) EIA Kit
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Type
Sandwich
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Description
The antagonist receptor ligand binding will be in contrast with agonist activity. Elabscience produces more antagonist and receptor related products as 1. This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
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Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Gene target
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Gene symbol
BAK1, BCL2
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Short name
BAK1(BCL2 Antagonist/Killer 1) EIA Kit
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Technique
EIA, eia
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Host
Rat
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Species
Rat, Rats
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Alternative name
Rat B-cellular CLL/lymphoma 2-anta this GO nist/killer 1(B-cellular CLL/lymphoma 2 Antagonist/Killer 1) EIA reagent
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Alternative technique
kits, elisas
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Alternative to gene target
B-cell CLL/lymphoma 2, Bcl-2 and PPP1R50, BCL2 and IDBG-4354 and ENSG00000171791 and 596, protein phosphatase 2A binding, nuclei, Bcl2 and IDBG-185673 and ENSMUSG00000057329 and 12043, BCL2 and IDBG-645006 and ENSBTAG00000019302 and 281020
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Gene info
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Identity
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Gene
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Long gene name
BCL2 antagonist/killer 1
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Synonyms gene
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Synonyms gene name
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Synonyms
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GenBank acession
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Locus
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Discovery year
1997-10-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
BCL2 apoptosis regulator
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Synonyms gene name
- B-cell CLL/lymphoma 2
- BCL2, apoptosis regulator
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- BCL2 family
- Protein phosphatase 1 regulatory subunits
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens.
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Tree numbers
- E05.478.566.350
- E05.478.583.400
- E05.601.470.350
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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