Recombinant Rat MMP-9 / CLG4B Protein (His tag)
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Catalog number
PKSR030381-10µg
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Price
Please ask
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Size
10µg
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Synonym
Mmp9
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Activity
Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076). The specific activity is >1000 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by APMA for an activated form)
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Sequence
Met 1-Pro 708
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Fusion tag
C-His
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Accession
EDL96479.1
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ExpressedHost
HEK293 Cells
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
> 95 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
77.8 kDa
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AP Mol Mass
100-110 KDa
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Formulation
Lyophilized from sterile 50mM MES, 100mM NaCl, 1mM CaCl2, 10% Glycerol, pH 7.4
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Background
Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions, such as morphogenesis, differentiation, angiogenesis and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases and tumor invasion. MMP9, also known as 92-kDa gelatinase B/type IV collagenase, is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity. It plays an important role in tissue remodelling in normal and pathological inflammatory processes. MMP-9 is a major secretion product of macrophages and a component of cytoplasmic granules of neutrophils, and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs including the lung. This enzyme is also secreted by lymphocytes and stromal cells upon stimulation by inflammatory cytokines, or upon delivery of bi-directional activation signals following integrin-mediated cell-cell or cell-extracellular matrix (ECM) contacts. Since the integrity of the tissue architecture is closely dependent of the delicate balance between MMPs and their inhibitors, excessive production of MMP-9 is linked to tissue damage and degenerative inflammatory disorders. As a consequence, regulation of gene transcription and tissue-specific expression of MMP-9 in normal and diseased states are being actively investigated to pave the way for new therapeutic targets. In addition, the dramatic overexpression of MMP-9 in cancer and various inflammatory conditions clearly points to the molecular mechanisms controlling its expression as a potential target for eventual rational therapeutic intervention.
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Conjugation
histidine
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MMP9, MMP24, MMP14, MMP28, RNU6-9, PIRC45, PIRC47
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Short name
Recombinant MMP-9 / CLG4B Protein (His tag)
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
Rat
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Label
His
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Species
Rat, Rats
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Alternative name
Rec. Rat MMP-9 / CLG4B Protein (histidine detection labelled)
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Alternative technique
rec
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Gene info
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
RNA, U6 small nuclear 9
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Synonyms
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Locus
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Discovery year
2008-06-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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