MHC Class 1, H2, Dd (FITC) Antibody

• Catalog number
  GEN608933
• Price
  Please ask
• Size
  500ug

• Also known as
  MHC Class 1, H2, Dd
• Other names
  N/A
• Category
  Antibodies
• Subcategory
  Mnoclonal antibodies
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Clonality
  Monoclonal
• Immunoglobulin isotype
  IgG2a,k
• Clone
  5K47
• Host organism
  Mouse (Mus musculus)
• Source organism
  Mouse (Mus musculus)
• Species reactivity
  Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
• Specificity and cross reactivity
  Recognizes a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro and cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed Has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
• Purification method
  Purified
• Form Appearance
  Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to FITC.
• Concentration
  N/A
• Storage and shipping
  Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested applications
  Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Properties
  If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  MHC   Class  
• Short name
  MHC Class 1, H2, Dd (FITC) Antibody
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  Anti-MHC Class 1, H2, Dd (FITC)
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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