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Also known as
ICAM3 / CD50
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Other names
intercellular adhesion molecule 3; Intercellular adhesion molecule 3; intercellular adhesion molecule 3; ICAM-3; intercellular adhesion molecule-3; intercellular adhesion molecule 3; CDw50; ICAM-R; CD_antigen: CD50
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
ICAM3
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Gene name synonims
CD50
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Other gene names
ICAM3; ICAM3; CD50; CDW50; ICAM-R; ICAM-3
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
MEM-04
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Host organism
Mouse (Mus musculus)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Reacts with CD50 (ICAM-3), a 120-130 kD type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes. The MEM-04 blocks DC-SIGN (CD209) binding to ICAM-3 (epitope in D1).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Protein A Purified
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Form Appearance
Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 mm filter sterilized.
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Concentration
1 miligram per 1 mililiter
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Storage and shipping
Store at +4 degrees Celsius for short term storage. For long term storage aliquot and keep the antibody at -20 degrees Celsius. Freeze-thaw cycles should be avoided in order to retain the activity of the antibody.
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Tested applications
Immunohistochemistry (IHC - Paraffin), Flow Cytometry (FC/FACS), Functional Assay
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
ICAM3, YARS2, HCG4P4
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Short name
Mouse Monoclonal [clone MEM-04] (IgG1) ICAM3 / CD50 Antibody
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Technique
Mouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Host
mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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Isotype
IgG1
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Species
Human, Humans, Mouses
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Alternative name
Anti-Mouse Monoclonal [clone MEM-04] (IgG1) to Human ICAM3 / CD50
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Alternative technique
antibodies, murine
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Alternative to gene target
intercellular adhesion molecule 3, CD50 and CDW50 and ICAM-R, ICAM3 and IDBG-27123 and ENSG00000076662 and 3385, protein binding, Plasma membranes, ICAM3 and IDBG-643267 and ENSBTAG00000015742 and 281840
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Clone name
MEM-04
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