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Stock availability
In Stock
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Scientific context
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
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Protein target
HSC70
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Protein reactivity
Human
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Certificate of analysis
This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.2µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
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Protein description
Active Human Recombinant HSC70 Protein
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Other name
Constitutive heat shock protein 70 Protein, HSC54 Protein, HSC71 Protein, HSC73 Protein, HSP71 Protein, HSP74 Protein, HSPA10 Protein, HSPA8 Protein, LAP1 Protein, NIP71 Protein
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Primary research area
Cancer, Heat Shock
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Category
Protein
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Brand name
none
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Origin
Recombinant
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NCBI number
AF352832
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Gene number
3312
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Protein number
P11142
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Verified applications
WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA
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Relevant bio activity
ATPase active
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Protein expression model
E. coli
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Protein charasterics
See included datasheet.
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Peptide sequence
See included datasheet.
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Protein purification
Affinity Purified
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Purity pourcentage
>90% High purity
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Recommended buffer for storage
20mM Tris/HCl pH7.5, 1mM EDTA
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Protein concentration
Lot/batch specific. See included datasheet.
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Protein specificity
~70 kDa
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Protein tag
His tag
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Supplementary useful information
Please see included datasheet or contact us
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Protein cell localization
Cytoplasm, Melanosome
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Bibliography
1. Brown C. L., et al. (1993) J.Cell Biol. 120(5): 1101-1112 2. Boorstein W. R., Ziegelhoffer T. & Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17. 3. Rothman J. (1989) Cell. 59: 591 -601. 4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887. 5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294. 6. Brown C. L., et al. (1996) J. Biol. Chem. 271(2): 833-840.
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Release date
1-Sep-2007
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PubMed number
28026090, 26244546, 25939977, 25979775, 23291500, 20084527, 19209902
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Tested applications
Western Blot, Functional Assay, Intrinsic fluorescence spectroscopy, Western Blot Control, Functional Assay, Functional Assay, Western Blot Control
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Tested species reactivity
Human, Mouse, Photinus pyralis
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Representative figure legend
SDS-PAGE of 73kDa Hsc70 protein (SPR-106). SDS-Page of human HSC70 Protein (SPR-106)
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Warnings
Non-hazardous materials
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Protein origin
Canada
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Total weight kg
1.4
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Net weight g
0.2
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants